Sbrasiliensis5110 – MATPredict report

Result

Mating type

MAT1-2: locus, medium confidence, 9.5 kb on AWTV01000010. Gene order: MAT1-2-1 · SLA2.

Called loci 1

MAT1-2 MAT family · Ascomycota

medium confidence

Gene order on AWTV01000010. Scroll sideways to see the whole figure.

FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.

core MAT gene
conserved flank
search hit only

MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.

Location: AWTV01000010:1,610,857–1,620,403 · 9.5 kb
Own genes: AWTV01000010:1,610,857–1,620,403 · 9.5 kb
Locus: Provisional: the call only tied the admission bar, or came from the relaxed pass.
Genes modelled: 2 of 3 gene hits
Nearest contig end: 1.6 Mb
Region (samtools): AWTV01000010:1610857-1620403

Idiomorph scores

MAT1-2 234.0

Margin over the set-aside MAT1-1-3 hit: 27.3 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

kept	identity %	set aside	identity %	overlap %	basis
MAT1-2-1	62.0	MAT1-1-3	34.7	100.0	higher identity

Gene evidence

Gene	Role	Model	Identity %	E-value	Position (strand)	Exons	Reference record	FungiDB gene
MAT1-1-3	core MATother idiomorph, not countedlost to MAT1-2-1, not counted	hit onlyDIAMOND, proteome	34.7cov 40.0%	9.9e-1665 bits	1,610,857–1,611,538 -	–	230073_uamh-1363_MAT_MAT1-1	SPBR_08948
MAT1-2-1	core MAT	hit onlyDIAMOND, proteome	68.6cov 26.6%	1.5e-80234 bits	1,611,588–1,611,803 -	–	230073_uamh-11059_MAT_MAT1-2	SPBR_05447
SLA2	conserved flank	hit onlyDIAMOND, proteome	93.9cov 100.0%	< 1e-1801,181 bits	1,616,939–1,620,403 -	–	230073_uamh-1363_MAT_MAT1-1	SPBR_04592
APN2	expected, not found							–
COX13	expected, not found							–

What was searched

Routing: By taxonomy. The genome's taxonomy falls inside a curated family's scope; only those families were searched.
Families searched: Ascomycota:MAT
Genetic code: 1
Taxonomy: local NCBI taxonomy snapshot 2026-10-01

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: MAT hit outside the flanking genes' span (1); too few of the family's genes found (1).

Show the 2 withheld loci

Withheld loci (2)

family	location	idiomorph	genes	modelled	reason
MAT	AWTV01000010:1,561,495–1,585,337	MAT1-1	MAT1-1-1, APN2, COX13	2	MAT hit outside the flanking genes' span
MAT	AWTV01000007:1,629,493–1,635,541	MAT1-1	MAT1-1-3, MAT1-2-7	1	too few of the family's genes found

Provenance

Genome file: Sbrasiliensis5110.genome.fasta
Assembly: 13 contigs · 33.2 Mb · N50 3.8 Mb
Genome SHA-256: f0bc39e5612c1318f32f98e556e652daf09a405038e60fe6a351267274882aa0
MATPredict: 0.8.1 (v0.8.1, 3a62e6302b)
Database: 86f18d232e4fe95d · 185 records
Taxonomy: local NCBI taxonomy snapshot 2026-10-01
Parameters: phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 5152, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 5152', 'routed_by': 'family taxid 5152 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started: 2026-11-07T02:33:17Z
Run time: 105 s
Files: detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph: The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence: Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence: The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence: A single isolated gene hit, or no core MAT gene.
Model status: Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity: Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record: The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits

MATPredict reads an assembly; it is not a mating assay. Two idiomorphs in one assembly can come from a homothallic species, a mixed culture or a merged diploid assembly.
Fragmented assemblies can split a locus or lose its flanking genes; an uncalled genome is weak evidence of absence.
Lineages with no curated record are searched with the whole phylum's references; family labels there are less reliable.