MATPredict · mating-type report

RmicrosporusATCC52814

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:53 UTC by MATPredict 0.8.1

Result

Mating type

Minushigh confidence

Called loci 1

Minus MAT family · Mucoromycota

high confidence
Gene order of the Minus locus on KV922032Minus locus: genes from left to right on KV922032, 10,201 to 19,021: glrA (variable flank, plus strand); rnhA (conserved flank, minus strand); sexM (core MAT gene, minus strand); sexP (core MAT gene, minus strand).10 kb12 kb14 kb16 kb18 kb← 9.8 kb more to contig endcalled locusglrA: variable flank, 10,201–11,754 (+), identity 98.3%, search hit onlyglrArnhA: conserved flank, 14,493–18,370 (-), identity 92.9%, search hit onlyrnhAsexM: core MAT gene, 18,434–18,880 (-), identity 93.2%, search hit onlysexMsexP: core MAT gene, 18,641–18,916 (-), identity 34.78%, gene modelsexP (lost to sexM)
Gene order on KV922032. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KV922032, 9,760 to 19,462BCV72DRAFT_43735 7,617-10,180 (+): SacI homology domain | domain-containing proteinBCV72DRAFT_43735BCV72DRAFT_43741 10,182-11,914 (+): Pyridine nucleotide-disulphide oxidoreductase | Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain | domain-containing proteinBCV72DRAFT_43741BCV72DRAFT_234133 12,112-13,971 (-): unspecified productBCV72DRAFT_234133BCV72DRAFT_234153 14,122-18,413 (-): P-loop containing nucleoside triphosphate hydrolase proteinBCV72DRAFT_234153BCV72DRAFT_234155 18,390-19,052 (-): HMG (high mobility group) box | domain-containing proteinBCV72DRAFT_234155
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • variable flank
  • gene model
  • search hit only
  • sexP is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KV922032:10,201–19,021 · 8.8 kb
Own genes
KV922032:10,201–18,916 · 8.7 kb · the span runs 105 bp beyond these genes
Supported span
KV922032:10,201–18,916 · 8.7 kb · 105 bp of the reported span rests only on hits below 33 bits
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
3 of 4 gene hits
Nearest contig end
10.2 kb
Region (samtools)
KV922032:10201-19021
Idiomorph scores
  • Minus 140.6
  • Plus 62.7

Margin over the next idiomorph: 77.9

Score: HMM bitscore of the MAT protein against each idiomorph's profile.

Profile-HMM classifier
  • Minus 140.6
  • Plus 62.7

Margin 77.9 (a call needs 25.0 or more). Scored: sexP (model).

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
sexM93.2sexP34.487.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
glrAvariable flankhit onlyDIAMOND, proteome98.3cov 100.0%< 1e-180904 bits10,201–11,754 +–86630_cbs357-93_MAT_MinusBCV72DRAFT_43741
rnhAconserved flankhit onlyDIAMOND, proteome92.9cov 93.3%< 1e-1802,146 bits14,493–18,370 -–86630_cbs357-93_MAT_MinusBCV72DRAFT_234153
sexMcore MAThit onlyDIAMOND, proteome93.2cov 79.5%1.7e-94264 bits18,434–18,880 -–86630_cbs357-93_MAT_MinusBCV72DRAFT_234155
sexPcore MATother idiomorph, not countedlost to sexM, not countedgene modelexonerate34.8cov 30.3%2.8e-0958 bits18,641–18,916 -13108396_ai-2023a_MAT_PlusBCV72DRAFT_234155
tptAexpected, not found–
algAexpected, not found–

Withheld candidate loci 13

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (12); MAT hit outside the flanking genes' span (1).

Show the 13 withheld loci

Withheld loci (13)

familylocationidiomorph genesmodelledreason
MATKV921866:15,833–89,855undeterminedtptA, sexM, algA2MAT hit outside the flanking genes' span
MATKV921862:48,884–55,021undeterminedrnhA1too few of the family's genes found
MATKV921866:204,526–205,062undeterminedsexM1too few of the family's genes found
MATKV921878:84,675–85,838undeterminedsexM1too few of the family's genes found
MATKV921881:47,292–48,479undeterminedtptA1too few of the family's genes found
MATKV921884:12,846–14,669undeterminedglrA1too few of the family's genes found
MATKV921890:4,727–10,615undeterminedrnhA1too few of the family's genes found
MATKV921925:23,029–24,385undeterminedsexP1too few of the family's genes found
MATKV921938:3,274–33,231undeterminedsexP, rnhA1too few of the family's genes found
MATKV921959:49,497–50,556undeterminedtptA1too few of the family's genes found
MATKV921984:16,941–20,373undeterminedrnhA1too few of the family's genes found
MATKV922059:13,817–18,117undeterminedbtbA1too few of the family's genes found
MATKV922082:2,249–3,713undeterminedglrA1too few of the family's genes found

Provenance

Genome file
RmicrosporusATCC52814.genome.fasta
Assembly
560 contigs · 25.0 Mb · N50 105.5 kb
Genome SHA-256
fdd5dc5924e502ea66ffff67cc99eb2c06fafdd5f8674e8fbb6b7aa173d531f8
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Mucoromycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Mucoromycota', 'phylum_taxid': 1913637, 'deepest_rank': 'genus', 'routing_taxid': 1344955, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Mucoromycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1344955', 'routed_by': 'family taxid 1344955 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:52:54Z
Run time
27 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits