MATPredict · mating-type report

PmacacaeP2C

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:53 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

P PM family · Ascomycota

medium confidence
Gene order of the P locus on CM030734P locus: genes from left to right on CM030734, 163,887 to 174,093: matMi (core MAT gene, minus strand); matMc (core MAT gene, plus strand); matPi (core MAT gene, plus strand).164 kb166 kb168 kb170 kb172 kb174 kb← 163.4 kb more to contig endcalled locusmatMi: core MAT gene, 163,887–164,027 (-), identity 60.417%, search hit onlymatMimatMc: core MAT gene, 164,243–165,001 (+), identity 90.1%, search hit onlymatMcmatPi: core MAT gene, 173,398–174,093 (+), identity 95.7%, search hit onlymatPi
Gene order on CM030734. Scroll sideways to see the whole figure.
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
Location
CM030734:163,887–174,093 · 10.2 kb
Cross-lineage class
unassigned
Own genes
CM030734:163,887–174,093 · 10.2 kb
Locus
Genes of both idiomorphs at one locus.
Genes modelled
2 of 3 gene hits
Nearest contig end
163.9 kb
Region (samtools)
CM030734:163887-174093
Idiomorph scores
  • P 433.0
  • M 425.0

Margin over the next idiomorph: 8.0

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
matMicore MAThit onlytblastn60.4cov 63.0%8.0e-0742 bits163,887–164,027 -–42068_ru7_PM_combinedPMAC_001418 (opposite strand)
matMccore MAThit onlyDIAMOND, proteome90.1cov 100.0%1.1e-156425 bits164,243–165,001 +–42068_ru7_PM_combinedPMAC_001419
matPicore MAThit onlyDIAMOND, proteome95.7cov 100.0%1.4e-160433 bits173,398–174,093 +–42068_ru7_PM_combinedPMAC_001421
matPcnot searchable: no reference protein in the database–

Withheld candidate loci 3

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (3).

Show the 3 withheld loci
familylocationidiomorph genesmodelledreason
PMCM030722:76,454–76,561MmatMi0fewer than 2 genes could be modelled
PMCM030725:157,060–157,164MmatMi0fewer than 2 genes could be modelled
PMCM030721:390,879–391,025MmatMi0fewer than 2 genes could be modelled

Provenance

Genome file
PmacacaeP2C.genome.fasta
Assembly
19 contigs · 8.3 Mb · N50 505.4 kb
Genome SHA-256
ac1c4374711005082d211959f78207741d8f1928f8a1c81036f68925658957e5
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 44281, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 44281', 'routed_by': 'family taxid 44281 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:53:32Z
Run time
11 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits