MATPredict · mating-type report

EpasteurianusEp9510

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:33 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1high confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on LGRN01000028MAT1-1 locus: genes from left to right on LGRN01000028, 21,777 to 46,402: MAT1-2-7 (core MAT gene, minus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand); MAT1-1-4 (core MAT gene, plus strand); MAT1-1-1 (core MAT gene, plus strand); SLA2 (conserved flank, minus strand).25 kb30 kb35 kb40 kb45 kb← 20.5 kb more to contig endcalled locusMAT1-2-7: core MAT gene, 21,777–21,887 (-), identity 35.135%, search hit onlyMAT1-2-7APN2: conserved flank, 29,001–31,117 (-), identity 68.9%, search hit onlyAPN2COX13: conserved flank, 31,715–32,446 (+), identity 79.8%, search hit onlyCOX13MAT1-1-4: core MAT gene, 35,400–36,838 (+), identity 43.7%, search hit onlyMAT1-1-4MAT1-1-1: core MAT gene, 37,594–38,874 (+), identity 47.6%, search hit onlyMAT1-1-1SLA2: conserved flank, 42,124–46,402 (-), identity 91.4%, search hit onlySLA2
Gene order on LGRN01000028. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on LGRN01000028, 20,548 to 47,634AJ78_01293 21,050-26,525 (-): Transcription mediator complex subunit Med12 | SRB8 ARM-like domain | domain-containing proteinAJ78_01293AJ78_01294 29,001-31,117 (-): DNA-(apurinic or apyrimidinic site) endonuclease 2AJ78_01294AJ78_01295 31,715-32,446 (+): Cytochrome c oxidase subunit VIa | domain-containing proteinAJ78_01295AJ78_01296 33,259-34,248 (+): PAT complex subunit Asterix | domain-containing proteinAJ78_01296AJ78_01297 35,400-36,838 (+): unspecified productAJ78_01297AJ78_01298 37,594-38,874 (+): Mating-type protein MAT alpha 1 HMG-box | domain-containing proteinAJ78_01298AJ78_01299 42,124-46,402 (-): ANTH domain | I/LWEQ domain | domain-containing proteinAJ78_01299AJ78_01300 47,543-50,450 (-): Pleckstrin homology domain | domain-containing proteinAJ78_01300
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-2-7 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
LGRN01000028:21,777–46,402 · 24.6 kb
Own genes
LGRN01000028:21,777–46,402 · 24.6 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 6 gene hits
Nearest contig end
21.8 kb
Region (samtools)
LGRN01000028:21777-46402
Idiomorph scores
  • MAT1-1 311.0
  • MAT1-2 27.7

Margin over the next idiomorph: 283.3

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-2-7core MATother idiomorph, not countedhit onlytblastn35.1cov 17.0%6.2e+0028 bits21,777–21,887 -–5158_cbs114723_MAT_unswitchedAJ78_01293
APN2conserved flankhit onlyDIAMOND, proteome68.9cov 86.5%3.7e-212598 bits29,001–31,117 -–27339_unknown-1_MAT_MAT1-2AJ78_01294
COX13conserved flankhit onlyDIAMOND, proteome79.8cov 73.3%3.7e-47142 bits31,715–32,446 +–5501_rs_MAT_MAT1-2AJ78_01295
MAT1-1-4core MAThit onlyDIAMOND, proteome43.7cov 70.3%7.9e-44142 bits35,400–36,838 +–199306_rmscc1040_MAT_MAT1-1AJ78_01297
MAT1-1-1core MAThit onlyDIAMOND, proteome47.6cov 99.2%9.5e-106311 bits37,594–38,874 +–5501_h538-4_MAT_MAT1-1AJ78_01298
SLA2conserved flankhit onlyDIAMOND, proteome91.4cov 99.3%< 1e-1801,631 bits42,124–46,402 -–107463_unknown-1_MAT_MAT1-2AJ78_01299
MAT1-2-1expected, not found–

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATLGRN01000071:39,253–64,851MAT1-2MAT1-1-1, MAT1-2-11too few of the family's genes found
MATLGRN01000278:16,987–18,714MAT1-1MAT1-1-31too few of the family's genes found

Provenance

Genome file
EpasteurianusEp9510.genome.fasta
Assembly
1,643 contigs · 32.4 Mb · N50 45.9 kb
Genome SHA-256
1c6d14421a08372519670a8c6cecb680eda3536d363c0908c1a9a2b4f5939581
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 299071, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 299071', 'routed_by': 'family taxid 299071 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:32:20Z
Run time
56 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits