MATPredict · mating-type report

DrugosaCBS613

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:19 UTC by MATPredict 0.8.1

Result

Mating type

Amedium confidence

Called loci 1

A MTL family · Ascomycota

medium confidence
Gene order of the A locus on SWFT01000050A locus: genes from left to right on SWFT01000050, 289,606 to 299,182: MTLA2 (core MAT gene, minus strand); PIK1 (variable flank, minus strand); OBP1 (variable flank, minus strand); PAP1 (variable flank, plus strand).290 kb292 kb294 kb296 kb298 kb202.6 kb more to contig end →called locusMTLA2: core MAT gene, 289,624–289,809 (-), identity 33.87%, gene modelMTLA2PIK1: variable flank, 290,127–292,838 (-), identity 54.8%, search hit onlyPIK1OBP1: variable flank, 293,043–294,365 (-), identity 69.2%, search hit onlyOBP1PAP1: variable flank, 295,932–299,182 (+), identity 68.2%, search hit onlyPAP1
Gene order on SWFT01000050. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on SWFT01000050, 289,129 to 299,660DIURU_001637 289,489-290,016 (-): unspecified productDIURU_001637DIURU_001638 290,127-292,838 (-): Phosphatidylinositol 3- and 4-kinase | Phosphoinositide 3-kinase family, accessory domain (PIK domain) | Yeast phosphatidylinositol-4-OH kinase Pik1 | domain-containing proteinDIURU_001638DIURU_001639 293,043-294,365 (-): Oxysterol-binding protein | domain-containing proteinDIURU_001639DIURU_001640 295,932-299,182 (+): Poly(A) polymerase nucleotidyltransferase domain | Poly(A) polymerase central domain | 60S ribosome biogenesis protein Rrp14 | Poly(A) polymerase predicted RNA binding domain | Surfeit locus protein 6 | domain-containing proteinDIURU_001640DIURU_001641 299,378-299,911 (+): TB2/DP1, HVA22 family | domain-containing proteinDIURU_001641
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • variable flank
  • gene model
  • search hit only
Location
SWFT01000050:289,606–299,182 · 9.6 kb
Cross-lineage class
MAT1-2
Own genes
SWFT01000050:289,624–299,182 · 9.6 kb · the span runs 18 bp beyond these genes
Locus
Provisional: the call only tied the admission bar, or came from the relaxed pass.
Genes modelled
4 of 4 gene hits
Nearest contig end
203.1 kb
Region (samtools)
SWFT01000050:289606-299182
Idiomorph scores
  • A 57.4

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MTLA2core MATgene modelexonerate33.9cov 33.0%9.0e-1057 bits289,624–289,809 -1717740_makgeolli_MTL_ADIURU_001637
PIK1variable flankhit onlyDIAMOND, proteome54.8cov 98.7%< 1e-180913 bits290,127–292,838 -–5476_sc5314_MTL_ADIURU_001638
OBP1variable flankhit onlyDIAMOND, proteome69.2cov 99.5%1.5e-224615 bits293,043–294,365 -–717740_makgeolli_MTL_ADIURU_001639
PAP1variable flankhit onlyDIAMOND, proteome68.2cov 97.8%1.3e-269756 bits295,932–299,182 +–5476_sc5314_MTL_ADIURU_001640
MTLA1expected, not found–

Withheld candidate loci 50

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (46); too few of the family's genes found (4).

Show the 50 withheld loci

Withheld loci (50)

familylocationidiomorph genesmodelledreason
MTLSWFT01000005:141,197–141,643alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000050:36,333–36,593AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000050:480,236–480,346AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000092:196,048–196,293AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000105:638,189–638,305alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000105:697,487–707,936AMTLA2, MTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000158:16,280–16,684alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000158:197,191–197,325AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000119:8,281–13,215alphaMTLA1, MTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000056:27,994–28,251alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000159:58,666–58,917AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000053:112,301–112,444AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000004:48,966–52,547AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000004:77,976–78,158AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000149:219,656–219,769AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000149:420,444–420,674alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000149:632,989–633,084AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000149:723,571–723,693AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000162:48,017–48,136AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000162:74,661–74,843AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000116:72,609–72,830AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000027:3,496–3,708AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000027:189,222–208,690alphaMTLalpha1, MTLalpha20fewer than 2 genes could be modelled
MTLSWFT01000037:3,835–4,068alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000038:86,830–86,970alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000039:29,834–29,998AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000039:87,039–87,134alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000031:45,037–45,222alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000139:45,081–45,182alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000032:106–261alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000090:58,384–58,629alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000090:149,983–150,210alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000090:175,534–175,623AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000018:101,072–101,302alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000018:142,875–143,030alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000064:70,751–71,092AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000064:204,162–204,308AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000098:22,025–22,159AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000098:64,713–64,940AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000122:40,722–40,874alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000107:88,077–88,217alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000044:52,531–52,626alphaMTLalpha10fewer than 2 genes could be modelled
MTLSWFT01000165:12,036–12,251AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000120:155,134–155,427AMTLA20fewer than 2 genes could be modelled
MTLSWFT01000163:226,612–226,752AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000163:255,520–255,717AMTLA10fewer than 2 genes could be modelled
MTLSWFT01000005:76,663–101,290alphaMTLalpha2, PIK11too few of the family's genes found
MTLSWFT01000092:69,636–70,892undeterminedOBP11too few of the family's genes found
MTLSWFT01000102:122,855–125,752undeterminedPIK11too few of the family's genes found
MTLSWFT01000105:587,496–588,104alphaMTLalpha21too few of the family's genes found

Provenance

Genome file
DrugosaCBS613.genome.fasta
Assembly
169 contigs · 13.4 Mb · N50 193.1 kb
Genome SHA-256
3203fd2444d83925ac66457557ca8dc41e76b511bf25398ef348f501e58997cb
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 766764, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 12, 'genetic_code_from': 'family taxid 766764', 'routed_by': 'family taxid 766764 (lineage)'}, genetic_code=12, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:19:26Z
Run time
22 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits