MATPredict · mating-type report

CyegresiiCBS114405

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:18 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-2 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on AMGW01000007MAT1-2 locus: genes from left to right on AMGW01000007, 1,417,656 to 1,442,459: MAT1-2-1 (core MAT gene, plus strand); MAT1-1-1 (core MAT gene, plus strand); MAT1-1-3 (core MAT gene, plus strand); APN2 (conserved flank, plus strand).1,420 kb1,425 kb1,430 kb1,435 kb1,440 kb← 1,416.4 kb more to contig endcalled locusMAT1-2-1: core MAT gene, 1,417,656–1,418,960 (+), identity 59.3%, search hit onlyMAT1-2-1MAT1-1-1: core MAT gene, 1,418,112–1,418,276 (+), identity 36.364%, search hit onlyMAT1-1-1 (lost to MAT1-2-1)MAT1-1-3: core MAT gene, 1,418,139–1,418,360 (+), identity 35.135%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)APN2: conserved flank, 1,440,206–1,442,459 (+), identity 64.5%, search hit onlyAPN2
Gene order on AMGW01000007. Scroll sideways to see the whole figure.
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-1 is a weak hit to the other idiomorph's gene and was not counted against the call.
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
AMGW01000007:1,417,656–1,442,459 · 24.8 kb
Own genes
AMGW01000007:1,417,656–1,442,459 · 24.8 kb
Locus
Provisional: the call only tied the admission bar, or came from the relaxed pass.
Genes modelled
2 of 4 gene hits
Nearest contig end
1.4 Mb
Region (samtools)
AMGW01000007:1417656-1442459
Idiomorph scores
  • MAT1-2 140.0

Margin over the set-aside MAT1-1-1 hit: 22.9 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-159.3MAT1-1-136.4100.0higher identity
MAT1-2-159.3MAT1-1-335.1100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-2-1core MAThit onlyDIAMOND, proteome59.3cov 23.1%1.0e-40140 bits1,417,656–1,418,960 +–1047171_ipo94269_MAT_MAT1-2A1O7_09629
MAT1-1-1core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlytblastn36.4cov 13.0%1.5e-0134 bits1,418,112–1,418,276 +–5127_mo44_MAT_MAT1-1A1O7_09629
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlytblastn35.1cov 41.0%5.3e-0954 bits1,418,139–1,418,360 +–230073_uamh-1363_MAT_MAT1-1A1O7_09629
APN2conserved flankhit onlyDIAMOND, proteome64.5cov 50.0%4.3e-183530 bits1,440,206–1,442,459 +–2903220_liq80xsp_MAT_combinedA1O7_09636
SLA2expected, not found–
COX13expected, not found–

Withheld candidate loci 4

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3); fewer than 2 genes could be modelled (1).

Show the 4 withheld loci
familylocationidiomorph genesmodelledreason
MATAMGW01000007:1,383,579–1,388,170MAT1-1MAT1-1-1, SLA21fewer than 2 genes could be modelled
MATAMGW01000001:736,684–762,890MAT1-2MAT1-1-1, MAT1-2-1, MAT1-2-71too few of the family's genes found
MATAMGW01000004:2,128,448–2,130,006MAT1-2MAT1-2-11too few of the family's genes found
MATAMGW01000007:1,281,172–1,293,326MAT1-1COX13, MAT1-1-21too few of the family's genes found

Provenance

Genome file
CyegresiiCBS114405.genome.fasta
Assembly
8 contigs · 27.9 Mb · N50 4.3 Mb
Genome SHA-256
4108f16fdf68f52c0a29300c47f8d48dbe2dfc165d9262ca5cfc38c41f662941
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 43219, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 43219', 'routed_by': 'family taxid 43219 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:17:41Z
Run time
58 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits