MATPredict · mating-type report

Cpurpurea20.1

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:14 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1high confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on CAGA01000045MAT1-1 locus: genes from left to right on CAGA01000045, 112,078 to 135,390: SLA2 (conserved flank, plus strand); MAT1-1-1 (core MAT gene, minus strand); MAT1-1-2 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).115 kb120 kb125 kb130 kb135 kb← 110.9 kb more to contig endcalled locusSLA2: conserved flank, 112,078–115,507 (+), identity 75.0%, search hit onlySLA2MAT1-1-1: core MAT gene, 126,350–127,639 (-), identity 46.3%, search hit onlyMAT1-1-1MAT1-1-2: core MAT gene, 128,635–129,117 (-), identity 36.02%, gene modelMAT1-1-2MAT1-1-3: core MAT gene, 129,868–130,540 (+), identity 44.05%, gene modelMAT1-1-3MAT1-2-1: core MAT gene, 130,265–130,567 (+), identity 33.663%, search hit onlyMAT1-2-1 (lost to MAT1-1-3)APN2: conserved flank, 131,813–133,887 (-), identity 77.3%, search hit onlyAPN2COX13: conserved flank, 134,706–135,390 (+), identity 66.0%, search hit onlyCOX13
Gene order on CAGA01000045. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on CAGA01000045, 110,913 to 136,554CPUR_06567 112,078-115,507 (+): I/LWEQ domain | ANTH domain | domain-containing proteinCPUR_06567CPUR_06568 126,350-127,639 (-): Mating-type protein MAT alpha 1 HMG-box | domain-containing proteinCPUR_06568CPUR_06569 131,813-133,887 (-): DNA-(apurinic or apyrimidinic site) endonuclease 2CPUR_06569CPUR_06570 134,706-135,390 (+): Cytochrome c oxidase subunit VIa | domain-containing proteinCPUR_06570CPUR_06571 136,130-138,657 (+): Anaphase-promoting complex subunit 5 | domain-containing proteinCPUR_06571
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • gene model
  • search hit only
  • MAT1-2-1 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
CAGA01000045:112,078–135,390 · 23.3 kb
Own genes
CAGA01000045:112,078–135,390 · 23.3 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
6 of 7 gene hits
Nearest contig end
112.1 kb
Region (samtools)
CAGA01000045:112078-135390
Idiomorph scores
  • MAT1-1 154.0

Margin over the set-aside MAT1-2-1 hit: 8.2 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-1-341.8MAT1-2-133.789.1higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome75.0cov 97.2%< 1e-1801,365 bits112,078–115,507 +–578113_sxlc146_MAT_MAT1-2CPUR_06567
MAT1-1-1core MAThit onlyDIAMOND, proteome46.3cov 13.4%5.4e-46154 bits126,350–127,639 -–230073_uamh-1363_MAT_MAT1-1CPUR_06568
MAT1-1-2core MATgene modelexonerate36.0cov 39.5%8.3e-29119 bits128,635–129,117 -15127_mo44_MAT_MAT1-1none
MAT1-1-3core MATgene modelexonerate44.0cov 95.6%3.5e-2087 bits129,868–130,540 +45518_3639_MAT_combinednone
MAT1-2-1core MATother idiomorph, not countedlost to MAT1-1-3, not countedhit onlytblastn33.7cov 21.0%3.1e-1062 bits130,265–130,567 +–5180_44ba1_MAT_combinednone
APN2conserved flankhit onlyDIAMOND, proteome77.3cov 36.7%4.2e-207586 bits131,813–133,887 -–2903220_liq80xsp_MAT_combinedCPUR_06569
COX13conserved flankhit onlyDIAMOND, proteome66.0cov 67.1%3.4e-49147 bits134,706–135,390 +–199306_rmscc1040_MAT_MAT1-1CPUR_06570

Withheld candidate loci 1

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (1).

Show the 1 withheld locus

Withheld loci (1)

familylocationidiomorph genesmodelledreason
MATCAGA01000050:94,095–96,436MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
Cpurpurea20.1.genome.fasta
Assembly
191 contigs · 32.1 Mb · N50 433.2 kb
Genome SHA-256
3b3ee82c27f5e5a571504850cfad68d6537060d340bf7fec3eb11f9bb4b6b9df
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 34397, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 34397', 'routed_by': 'family taxid 34397 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:13:05Z
Run time
65 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits