MATPredict · mating-type report

CposadasiiC735deltSOWgp

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:21 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2high confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on ACFW01000049MAT1-2 locus: genes from left to right on ACFW01000049, 4,371,761 to 4,384,013: SLA2 (conserved flank, plus strand); MAT1-2-4 (core MAT gene, minus strand); CIMG_00407 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).4,372 kb4,374 kb4,376 kb4,378 kb4,380 kb4,382 kb4,384 kb1,013.7 kb more to contig end →called locusSLA2: conserved flank, 4,371,761–4,375,696 (+), identity 99.8%, search hit onlySLA2MAT1-2-4: core MAT gene, 4,377,590–4,377,712 (-), identity 34.146%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 4,377,811–4,378,350 (-), identity 98.9%, search hit onlyCIMG_00407MAT1-1-3: core MAT gene, 4,378,795–4,379,924 (+), identity 26.3%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 4,378,795–4,379,924 (+), identity 100.0%, search hit onlyMAT1-2-1APN2: conserved flank, 4,380,656–4,382,712 (-), identity 91.1%, search hit onlyAPN2COX13: conserved flank, 4,383,345–4,384,013 (+), identity 98.5%, search hit onlyCOX13
Gene order on ACFW01000049. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on ACFW01000049, 4,371,149 to 4,384,625CPC735_058010 4,368,326-4,371,224 (+): Pleckstrin homology domain | Maintenance of mitochondrial morphology protein 1 | domain-containing proteinCPC735_058010CPC735_058020 4,371,565-4,376,644 (+): endocytosis protein end4, putativeCPC735_058020CPC735_058030 4,377,811-4,378,350 (-): hypothetical proteinCPC735_058030CPC735_058040 4,378,671-4,380,292 (+): mating type HMG-box protein, putativeCPC735_058040CPC735_058050 4,380,656-4,382,712 (-): DNA lyase Apn2, putativeCPC735_058050CPC735_058060 4,383,291-4,384,239 (+): cytochrome c oxidase polypeptide VIa, mitochondrial precursor, putativeCPC735_058060CPC735_058070 4,384,390-4,385,968 (-): Mannosyltransferase putative | domain-containing proteinCPC735_058070
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
ACFW01000049:4,371,761–4,384,013 · 12.3 kb
Own genes
ACFW01000049:4,371,761–4,384,013 · 12.3 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
1.0 Mb
Region (samtools)
ACFW01000049:4371761-4384013
Idiomorph scores
  • MAT1-2 663.0

Margin over the set-aside MAT1-1-3 hit: 73.7 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-1100.0MAT1-1-326.3100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome99.8cov 100.0%< 1e-1801,808 bits4,371,761–4,375,696 +–5501_rs_MAT_MAT1-2CPC735_058020
MAT1-2-4core MAThit onlytblastn34.1cov 16.0%9.4e-0736 bits4,377,590–4,377,712 -–746128_af293_MAT_MAT1-2none
CIMG_00407core MAThit onlyDIAMOND, proteome98.9cov 68.1%2.5e-124344 bits4,377,811–4,378,350 -–5501_rs_MAT_MAT1-2CPC735_058030
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome26.3cov 83.3%3.8e-1566 bits4,378,795–4,379,924 +–230073_uamh-1363_MAT_MAT1-1CPC735_058040
MAT1-2-1core MAThit onlyDIAMOND, proteome100.0cov 100.0%1.6e-246663 bits4,378,795–4,379,924 +–199306_silveira_MAT_MAT1-2CPC735_058040
APN2conserved flankhit onlyDIAMOND, proteome91.1cov 100.0%< 1e-1801,114 bits4,380,656–4,382,712 -–5501_rs_MAT_MAT1-2CPC735_058050
COX13conserved flankhit onlyDIAMOND, proteome98.5cov 100.0%2.0e-92256 bits4,383,345–4,384,013 +–5501_rs_MAT_MAT1-2CPC735_058060

Withheld candidate loci 1

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (1).

Show the 1 withheld locus

Withheld loci (1)

familylocationidiomorph genesmodelledreason
MATACFW01000049:1,658,398–1,660,605MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
CposadasiiC735deltSOWgp.genome.fasta
Assembly
55 contigs · 27.0 Mb · N50 2.4 Mb
Genome SHA-256
f483ac33af7cfcf4dd5d62c084a5f8034708c36b4a24d55bf83d7a0741a94cb8
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 33184, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 33184', 'routed_by': 'family taxid 33184 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:21:07Z
Run time
47 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits