MATPredict · mating-type report

CgattiiNT10

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:19 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

alpha MAT family · Basidiomycota

medium confidence
Gene order of the alpha locus on KN905664alpha locus: genes from left to right on KN905664, 39,402 to 45,817: STE3 (core MAT gene, plus strand); MFalpha (core MAT gene, minus strand); MFa (core MAT gene, minus strand).40 kb41 kb42 kb43 kb44 kb45 kbcontig endcalled locusSTE3: core MAT gene, 39,402–40,720 (+), identity 90.5%, search hit onlySTE3MFalpha: core MAT gene, 45,701–45,817 (-), identity 94.7%, search hit onlyMFalphaMFa: core MAT gene, 45,704–45,817 (-), identity 54.762%, search hit onlyMFa (lost to MFalpha)
Gene order on KN905664. Scroll sideways to see the whole figure.
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
Location
KN905664:39,402–45,817 · 6.4 kb
Own genes
KN905664:39,402–45,817 · 6.4 kb
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 3 gene hits
Nearest contig end
165 bp
Region (samtools)
KN905664:39402-45817
Idiomorph scores
  • alpha 72.0

Margin over the set-aside MFa hit: 39.9 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MFalpha94.7MFa54.8100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
STE3core MAThit onlyDIAMOND, proteome90.5cov 100.0%5.0e-262703 bits39,402–40,720 +–40410_jec21_MAT_alphaI311_06454
MFalphacore MAThit onlyDIAMOND, proteome94.7cov 100.0%3.1e-2372 bits45,701–45,817 -–40410_jec21_MAT_alphaI311_06456
MFacore MATlost to MFalpha, not countedhit onlytblastn54.8cov 100.0%2.2e-0640 bits45,704–45,817 -–40410_jec20_MAT_aI311_06456
FAO1expected, not found–
PAN6expected, not found–
NOG2not searchable: no reference protein in the database–
UAP1not searchable: no reference protein in the database–

Withheld candidate loci 5

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (5).

Show the 5 withheld loci
familylocationidiomorph genesmodelledreason
MATKN905586:357,922–359,350undeterminedSTE31too few of the family's genes found
MATKN905605:3,538–5,219undeterminedPAN61too few of the family's genes found
MATKN905629:88,820–92,676alphaFAO1, SXI1, SXI23too few of the family's genes found
MATKN905702:7,541–8,040undeterminedRPL391too few of the family's genes found
MATKN905707:166–10,761alphaMFalpha1too few of the family's genes found

Provenance

Genome file
CgattiiNT10.genome.fasta
Assembly
226 contigs · 18.0 Mb · N50 283.2 kb
Genome SHA-256
9d3c969ffe8cb5e0fa636db5b9d63096fa69ac8e94f94ee75e484781049c6b9d
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'genus', 'routing_taxid': 1884633, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1884633', 'routed_by': 'family taxid 1884633 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:19:15Z
Run time
25 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits