MATPredict · mating-type report

CbacillisporusCA1873

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:35 UTC by MATPredict 0.8.1

Result

Mating type

ahigh confidence

Called loci 1

a MAT family · Basidiomycota

high confidence
Gene order of the a locus on KN848897a locus: genes from left to right on KN848897, 134,584 to 253,284: SXI2 (core MAT gene, minus strand); RPL39 (core MAT gene, plus strand); STE3 (core MAT gene, minus strand); MFa (core MAT gene, plus strand); MFalpha (core MAT gene, plus strand); PAN6 (conserved flank, plus strand).140 kb160 kb180 kb200 kb220 kb240 kb← 128.6 kb more to contig endcalled locusSXI2: core MAT gene, 134,584–137,112 (-), identity 68.7%, search hit onlySXI2RPL39: core MAT gene, 155,342–155,597 (+), identity 100.0%, the two modelling tools disagreeRPL39STE3: core MAT gene, 215,238–216,570 (-), identity 64.6%, search hit onlySTE3MFa: core MAT gene, 241,431–241,553 (+), identity 95.12%, gene modelMFaMFalpha: core MAT gene, 241,431–241,553 (+), identity 56.098%, search hit onlyMFalpha (lost to MFa)PAN6: conserved flank, 251,592–253,284 (+), identity 90.5%, search hit onlyPAN6
Gene order on KN848897. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KN848897, 128,647 to 259,226I314_03609 128,417-129,165 (+): hypothetical proteinI314_03609I314_03610 132,073-133,933 (-): NCS2 family nucleobase:cation symporter-2I314_03610I314_03611 134,584-137,112 (-): Homeodomain | domain-containing proteinI314_03611I314_03612 138,253-138,591 (-): hypothetical proteinI314_03612I314_03613 140,121-144,538 (-): phospholipase DI314_03613I314_03614 146,006-148,244 (+): Glycosyl transferase family 90 | domain-containing proteinI314_03614I314_03615 151,954-152,603 (+): hypothetical proteinI314_03615I314_03616 157,573-159,344 (-): BTB/POZ domain | domain-containing proteinI314_03616I314_03617 162,977-166,984 (-): DNA-directed RNA polymerase, mitochondrialI314_03617I314_03618 167,602-170,318 (+): hypothetical proteinI314_03618I314_03619 170,739-171,446 (-): hypothetical proteinI314_03619I314_03620 173,023-175,973 (-): polynucleotide adenylyltransferaseI314_03620I314_03621 176,838-180,007 (-): RhoGEF domain | domain-containing proteinI314_03621I314_03622 181,144-181,767 (+): hypothetical proteinI314_03622I314_03623 183,035-185,786 (-): Structure-specific endonuclease subunit SLX4I314_03623I314_03624 192,052-192,456 (-): hypothetical proteinI314_03624I314_03625 194,580-195,178 (+): 50S small subunit ribosomal protein L22eI314_03625I314_03626 196,021-197,431 (+): electron transfer flavoprotein alpha subunitI314_03626I314_03627 199,165-199,539 (+): hypothetical proteinI314_03627I314_03628 199,752-204,635 (-): STE/STE11 protein kinaseI314_03628I314_03629 207,080-209,849 (+): eukaryotic translation initiation factor 3 subunit BI314_03629I314_03630 209,971-213,602 (-): BAH domain | PHD-finger | PHD-finger | domain-containing proteinI314_03630I314_03631 215,238-216,570 (-): Pheromone A receptor | domain-containing proteinI314_03631I314_03632 219,738-220,293 (-): hypothetical proteinI314_03632I314_03633 224,024-224,371 (+): hypothetical proteinI314_03633I314_03634 225,339-227,783 (+): STE/STE20/PAKA protein kinaseI314_03634I314_03635 230,854-232,502 (-): IKS protein kinaseI314_03635I314_03636 234,632-240,416 (+): IQ calmodulin-binding motif | Myosin head (motor domain) | DIL domain | domain-containing proteinI314_03636I314_03637 247,159-247,690 (+): hypothetical proteinI314_03637I314_03638 248,395-251,251 (+): nucleolar GTP-binding protein 2I314_03638I314_03639 251,592-253,284 (+): pantoate-beta-alanine ligaseI314_03639I314_03640 253,487-256,112 (-): hypothetical proteinI314_03640I314_03641 257,039-257,458 (-): Heat shock protein 9/12 | domain-containing proteinI314_03641I314_03642 257,543-257,683 (+): hypothetical proteinI314_03642I314_03643 258,479-261,542 (-): autophagy-like protein 9I314_03643
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • gene model
  • tools disagree
  • alternate model
  • search hit only
Location
KN848897:134,584–253,284 · 118.7 kb
Own genes
KN848897:134,584–253,284 · 118.7 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 6 gene hits
Nearest contig end
134.6 kb
Region (samtools)
KN848897:134584-253284
Idiomorph scores
  • a 881.0

Margin over the set-aside MFalpha hit: 24.9 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MFa81.0MFalpha56.1100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SXI2core MAThit onlyDIAMOND, proteome68.7cov 100.0%5.6e-322881 bits134,584–137,112 -–40410_jec20_MAT_aI314_03611
RPL39core MATmodels differexonerateminiprot: 155,342–155,600, 92.8%100.0cov 98.0%1.1e-1667 bits155,342–155,597 +340410_jec20_MAT_anone
STE3core MAThit onlyDIAMOND, proteome64.6cov 99.7%1.7e-163449 bits215,238–216,570 -–40410_jec20_MAT_aI314_03631
MFacore MATgene modelexonerate95.1cov 97.6%6.8e-1256 bits241,431–241,553 +140410_jec20_MAT_anone
MFalphacore MATlost to MFa, not countedhit onlytblastn56.1cov 100.0%2.6e-0537 bits241,431–241,553 +–40410_jec21_MAT_alphanone
PAN6conserved flankhit onlyDIAMOND, proteome90.5cov 100.0%7.1e-265712 bits251,592–253,284 +–40410_jec20_MAT_aI314_03639
FAO1expected, not found–
NOG2not searchable: no reference protein in the database–
UAP1not searchable: no reference protein in the database–

Provenance

Genome file
CbacillisporusCA1873.genome.fasta
Assembly
33 contigs · 17.4 Mb · N50 761.6 kb
Genome SHA-256
cf0f0251c31bb7d23b1e11d482d9a01dc995b786537ad49aedef02ce1119bba7
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'species', 'routing_taxid': 1884633, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1884633', 'routed_by': 'family taxid 1884633 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:35:00Z
Run time
20 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits