MATPredict · mating-type report

CaurisB8441

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:34 UTC by MATPredict 0.8.1

Result

Mating type

Ahigh confidence

Called loci 1

A MTL family · Ascomycota

high confidence
Gene order of the A locus on PEKT02000003A locus: genes from left to right on PEKT02000003, 845,925 to 853,762: PAP1 (variable flank, minus strand); OBP1 (variable flank, plus strand); PIK1 (variable flank, plus strand); MTLA2 (core MAT gene, plus strand); MTLA1 (core MAT gene, plus strand).846 kb848 kb850 kb852 kb854 kb193.3 kb more to contig end →called locusPAP1: variable flank, 845,925–847,559 (-), identity 100.0%, search hit onlyPAP1OBP1: variable flank, 847,786–849,075 (+), identity 100.0%, search hit onlyOBP1PIK1: variable flank, 849,738–852,365 (+), identity 100.0%, search hit onlyPIK1MTLA2: core MAT gene, 852,451–853,064 (+), identity 98.68%, the two modelling tools disagreeMTLA2MTLA1: core MAT gene, 853,144–853,762 (+), identity 100.0%, gene modelMTLA1
Gene order on PEKT02000003. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on PEKT02000003, 845,534 to 854,153B9J08_001452 844,401-845,882 (+): Insulinase (Peptidase family M16) | Peptidase M16 inactive domain | domain-containing proteinB9J08_001452B9J08_001453 845,925-847,559 (-): Poly(A) polymerase PAPaB9J08_001453B9J08_001454 847,786-849,075 (+): Oxysterol-binding protein | domain-containing proteinB9J08_001454B9J08_001455 849,738-852,365 (+): Phosphatidylinositol 3- and 4-kinase | Yeast phosphatidylinositol-4-OH kinase Pik1 | domain-containing proteinB9J08_001455B9J08_001456 853,714-856,101 (-): hypothetical proteinB9J08_001456
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • variable flank
  • gene model
  • tools disagree
  • alternate model
  • search hit only
Location
PEKT02000003:845,925–853,762 · 7.8 kb
Cross-lineage class
MAT1-2
Own genes
PEKT02000003:845,925–853,762 · 7.8 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 5 gene hits
Nearest contig end
193.7 kb
Region (samtools)
PEKT02000003:845925-853762
Idiomorph scores
  • A 290.0

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
PAP1variable flankhit onlyDIAMOND, proteome100.0cov 100.0%< 1e-1801,087 bits845,925–847,559 -–498019_b11205_MTL_AB9J08_001453
OBP1variable flankhit onlyDIAMOND, proteome100.0cov 100.0%7.0e-322860 bits847,786–849,075 +–498019_b11205_MTL_AB9J08_001454
PIK1variable flankhit onlyDIAMOND, proteome100.0cov 100.0%< 1e-1801,649 bits849,738–852,365 +–498019_b11205_MTL_AB9J08_001455
MTLA2core MATmodels differexonerateminiprot: 852,451–853,067, 92.5%98.7cov 100.0%3.1e-48164 bits852,451–853,064 +2498019_b11243_MTL_Anone
MTLA1core MATgene modelexonerate100.0cov 100.0%1.2e-91290 bits853,144–853,762 +3498019_b11205_MTL_AB9J08_001456 (opposite strand)

Withheld candidate loci 47

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (46); too few of the family's genes found (1).

Show the 47 withheld loci

Withheld loci (47)

familylocationidiomorph genesmodelledreason
MTLPEKT02000001:48,129–48,278alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000001:160,876–160,992alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000001:213,907–214,038alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000001:719,742–744,591alphaMTLA1, MTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000001:983,597–1,009,424AMTLA1, PIK11fewer than 2 genes could be modelled
MTLPEKT02000002:202,250–202,489AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000002:322,794–322,925AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000002:609,516–609,728alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000002:708,511–708,648AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000002:1,045,558–1,045,707AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000002:1,190,452–1,190,631AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000003:329,999–330,112AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000003:408,224–408,340alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000007:299,538–319,906AMTLA1, MTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000007:642,917–643,039AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000007:869,047–869,157AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000007:975,669–975,761AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000007:2,263,774–2,263,899AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000007:2,401,287–2,420,012AMTLA1, PIK11fewer than 2 genes could be modelled
MTLPEKT02000007:2,601,321–2,601,521alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000007:2,959,737–2,959,973AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000007:3,063,504–3,063,686AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000008:175,718–175,816alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000008:216,712–216,810alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000008:292,891–293,034AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000008:320,557–320,721alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000008:484,185–484,319alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000008:584,762–585,094AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000008:740,031–740,156AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000008:817,558–817,677AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000010:331,691–354,766AMTLA2, MTLalpha1, MTLalpha20fewer than 2 genes could be modelled
MTLPEKT02000010:480,467–480,571alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000010:513,197–513,400AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000010:632,927–633,034AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000010:981,875–982,006AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000010:1,323,684–1,323,869AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000005:312,630–315,596AMTLA1, MTLA20fewer than 2 genes could be modelled
MTLPEKT02000005:430,377–430,616alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000005:638,327–638,488AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000009:422,302–422,541AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000009:884,613–884,885AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000009:987,766–987,906AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000006:104,889–105,059alphaMTLalpha10fewer than 2 genes could be modelled
MTLPEKT02000006:216,604–216,783AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000004:673,250–673,366AMTLA10fewer than 2 genes could be modelled
MTLPEKT02000004:731,297–731,467AMTLA20fewer than 2 genes could be modelled
MTLPEKT02000002:805,981–807,240undeterminedOBP11too few of the family's genes found

Provenance

Genome file
CaurisB8441.genome.fasta
Assembly
15 contigs · 12.4 Mb · N50 1.1 Mb
Genome SHA-256
7f2d8baa6c4cda45a4bcb5d7fa484ed67d9759ac98f8dcf218d1dd941613f4b5
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 27319, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 12, 'genetic_code_from': 'family taxid 27319', 'routed_by': 'family taxid 27319 (lineage)'}, genetic_code=12, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:34:35Z
Run time
23 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits