MATPredict · mating-type report

AversicolorCBS583.65

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:41 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2medium confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on KV878127MAT1-2 locus: genes from left to right on KV878127, 2,870,310 to 2,889,382: COX13 (conserved flank, minus strand); APN2 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-2-4 (core MAT gene, plus strand); CIMG_00407 (core MAT gene, plus strand); SLA2 (conserved flank, minus strand).2,870 kb2,875 kb2,880 kb2,885 kb2,890 kb783.9 kb more to contig end →called locusCOX13: conserved flank, 2,870,310–2,873,341 (-), identity 89.1%, search hit onlyCOX13APN2: conserved flank, 2,873,650–2,875,779 (+), identity 88.6%, search hit onlyAPN2MAT1-1-3: core MAT gene, 2,880,609–2,881,665 (-), identity 39.7%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 2,880,609–2,881,665 (-), identity 71.1%, search hit onlyMAT1-2-1MAT1-2-4: core MAT gene, 2,882,201–2,883,148 (+), identity 46.0%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 2,882,787–2,882,927 (+), identity 42.308%, search hit onlyCIMG_00407SLA2: conserved flank, 2,885,494–2,889,382 (-), identity 89.5%, search hit onlySLA2
Gene order on KV878127. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KV878127, 2,869,357 to 2,890,334ASPVEDRAFT_128383 2,868,583-2,869,930 (-): Glycosyltransferase family 69 proteinASPVEDRAFT_128383ASPVEDRAFT_51798 2,870,310-2,873,341 (-): FAD-binding domain-containing proteinASPVEDRAFT_51798ASPVEDRAFT_82220 2,873,650-2,875,779 (+): DNA-(apurinic or apyrimidinic site) endonuclease 2ASPVEDRAFT_82220ASPVEDRAFT_40208 2,876,554-2,879,572 (-): Anaphase-promoting complex subunit 5 (Cyclosome subunit 5)ASPVEDRAFT_40208ASPVEDRAFT_82222 2,880,609-2,881,665 (-): HMG box domain-containing proteinASPVEDRAFT_82222ASPVEDRAFT_149602 2,882,056-2,883,148 (+): Mating locus proteinASPVEDRAFT_149602ASPVEDRAFT_40212 2,885,005-2,889,620 (-): Cytoskeleton assembly control protein Sla2ASPVEDRAFT_40212ASPVEDRAFT_128850 2,890,139-2,892,970 (-): SMP-LTD domain-containing proteinASPVEDRAFT_128850
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KV878127:2,870,310–2,889,382 · 19.1 kb
Own genes
KV878127:2,870,310–2,889,382 · 19.1 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
784.8 kb
Region (samtools)
KV878127:2870310-2889382
Idiomorph scores
  • MAT1-2 429.0

Margin over the set-aside MAT1-1-3 hit: 31.4 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-171.1MAT1-1-339.7100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
COX13conserved flankhit onlyDIAMOND, proteome89.1cov 88.1%3.7e-66206 bits2,870,310–2,873,341 -–162425_fgsc-a4_MAT_combinedASPVEDRAFT_51798
APN2conserved flankhit onlyDIAMOND, proteome88.6cov 36.7%< 1e-180936 bits2,873,650–2,875,779 +–2903220_liq80xsp_MAT_combinedASPVEDRAFT_82220
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome39.7cov 43.3%3.6e-1668 bits2,880,609–2,881,665 -–230073_uamh-1363_MAT_MAT1-1ASPVEDRAFT_82222
MAT1-2-1core MAThit onlyDIAMOND, proteome71.1cov 100.0%7.0e-155429 bits2,880,609–2,881,665 -–162425_fgsc-a4_MAT_combinedASPVEDRAFT_82222
MAT1-2-4core MAThit onlyDIAMOND, proteome46.0cov 95.6%5.0e-73217 bits2,882,201–2,883,148 +–746128_af293_MAT_MAT1-2ASPVEDRAFT_149602
CIMG_00407core MAThit onlytblastn42.3cov 20.0%1.7e-1247 bits2,882,787–2,882,927 +–5501_rs_MAT_MAT1-2ASPVEDRAFT_149602
SLA2conserved flankhit onlyDIAMOND, proteome89.5cov 100.0%< 1e-1801,565 bits2,885,494–2,889,382 -–1301501_voucher-215_MAT_MAT1-1ASPVEDRAFT_40212

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATKV878126:1,873,872–1,877,431MAT1-2MAT1-2-1, CIMG_004071too few of the family's genes found
MATKV878133:916,019–930,325MAT1-1MAT1-2-1, MAT1-1-32too few of the family's genes found

Provenance

Genome file
AversicolorCBS583.65.genome.fasta
Assembly
51 contigs · 33.1 Mb · N50 2.5 Mb
Genome SHA-256
a93c037729e6b5502a746f18dfbaf22e1877a28bd97eb838474564a89b0da161
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:40:39Z
Run time
73 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits