MATPredict · mating-type report

AdauciA2016

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:20 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on CM090138MAT1-2 locus: genes from left to right on CM090138, 2,838,460 to 2,845,128: COX13 (conserved flank, minus strand); APN2 (conserved flank, plus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, minus strand).2,840 kb2,842 kb2,844 kb596.9 kb more to contig end →called locusCOX13: conserved flank, 2,838,460–2,839,096 (-), identity 51.3%, search hit onlyCOX13APN2: conserved flank, 2,839,406–2,841,364 (+), identity 88.6%, search hit onlyAPN2MAT1-2-1: core MAT gene, 2,844,046–2,845,128 (-), identity 56.7%, search hit onlyMAT1-2-1MAT1-1-3: core MAT gene, 2,844,475–2,844,747 (-), identity 30.769%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)
Gene order on CM090138. Scroll sideways to see the whole figure.
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
CM090138:2,838,460–2,845,128 · 6.7 kb
Own genes
CM090138:2,838,460–2,845,128 · 6.7 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
3 of 4 gene hits
Nearest contig end
597.3 kb
Region (samtools)
CM090138:2838460-2845128
Idiomorph scores
  • MAT1-2 349.0

Margin over the set-aside MAT1-1-3 hit: 25.9 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-156.7MAT1-1-330.8100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
COX13conserved flankhit onlyDIAMOND, proteome51.3cov 82.2%1.5e-38120 bits2,838,460–2,839,096 -–5501_rs_MAT_MAT1-2ACET3X_005495
APN2conserved flankhit onlyDIAMOND, proteome88.6cov 36.7%3.2e-220621 bits2,839,406–2,841,364 +–2903220_liq80xsp_MAT_combinedACET3X_005496
MAT1-2-1core MAThit onlyDIAMOND, proteome56.7cov 92.7%2.2e-122349 bits2,844,046–2,845,128 -–5016_c4_MAT_MAT1-2ACET3X_005497
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlytblastn30.8cov 40.0%3.2e-0852 bits2,844,475–2,844,747 -–5518_3639_MAT_combinedACET3X_005497
SLA2expected, not found–

Withheld candidate loci 3

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3).

Show the 3 withheld loci
familylocationidiomorph genesmodelledreason
MATCM090138:2,759,078–2,762,591undeterminedSLA21too few of the family's genes found
MATCM090143:472,748–477,681MAT1-2MAT1-2-11too few of the family's genes found
MATCM090144:699,389–730,857MAT1-2MAT1-2-1, MAT1-1-21too few of the family's genes found

Provenance

Genome file
AdauciA2016.genome.fasta
Assembly
10 contigs · 34.9 Mb · N50 3.4 Mb
Genome SHA-256
842efb3bc415b39fd1c6653f7221ce1e9c3d99de015ad0c44e0e612416085c7b
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 28556, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 28556', 'routed_by': 'family taxid 28556 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:19:12Z
Run time
67 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits