MATPredict · mating-type report

AaculeatusATCC16872

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:12 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2high confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on KV878975MAT1-2 locus: genes from left to right on KV878975, 261,063 to 286,159: SLA2 (conserved flank, plus strand); MAT1-2-4 (core MAT gene, minus strand); CIMG_00407 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).260 kb265 kb270 kb275 kb280 kb285 kb← 259.8 kb more to contig endcalled locusSLA2: conserved flank, 261,063–264,927 (+), identity 89.6%, search hit onlySLA2MAT1-2-4: core MAT gene, 277,841–278,668 (-), identity 41.2%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 278,183–278,443 (-), identity 24.719%, search hit onlyCIMG_00407MAT1-1-3: core MAT gene, 279,215–280,281 (+), identity 36.5%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 279,215–280,281 (+), identity 61.8%, search hit onlyMAT1-2-1APN2: conserved flank, 281,543–284,859 (-), identity 90.9%, search hit onlyAPN2COX13: conserved flank, 285,291–286,159 (+), identity 78.8%, search hit onlyCOX13
Gene order on KV878975. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KV878975, 259,808 to 287,412ASPACDRAFT_77563 257,298-260,565 (+): SMP-LTD domain-containing proteinASPACDRAFT_77563ASPACDRAFT_117010 260,750-265,368 (+): Cytoskeleton assembly control protein Sla2ASPACDRAFT_117010ASPACDRAFT_117014 266,904-268,739 (-): FAD-binding domain-containing proteinASPACDRAFT_117014ASPACDRAFT_1899230 268,636-270,562 (-): CAIB/BAIF family enzymeASPACDRAFT_1899230ASPACDRAFT_1887453 271,080-273,163 (+): Aldehyde dehydrogenase domain-containing proteinASPACDRAFT_1887453ASPACDRAFT_51613 273,014-274,072 (-): HD/PDEase domain-containing proteinASPACDRAFT_51613ASPACDRAFT_9218 277,841-278,668 (-): Mating locus proteinASPACDRAFT_9218ASPACDRAFT_1867751 279,215-280,464 (+): HMG box domain-containing proteinASPACDRAFT_1867751ASPACDRAFT_117017 281,300-284,890 (-): DNA-(apurinic or apyrimidinic site) endonuclease 2ASPACDRAFT_117017ASPACDRAFT_42175 285,291-286,159 (+): Cytochrome c oxidase subunit (Cytochrome c oxidase polypeptide VIa)ASPACDRAFT_42175ASPACDRAFT_27181 286,627-289,303 (-): Anaphase-promoting complex subunit 5 (Cyclosome subunit 5)ASPACDRAFT_27181
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KV878975:261,063–286,159 · 25.1 kb
Own genes
KV878975:261,063–286,159 · 25.1 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
261.1 kb
Region (samtools)
KV878975:261063-286159
Idiomorph scores
  • MAT1-2 261.0

Margin over the set-aside MAT1-1-3 hit: 25.3 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-161.8MAT1-1-336.5100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome89.6cov 100.0%< 1e-1801,612 bits261,063–264,927 +–746128_af293_MAT_MAT1-2ASPACDRAFT_117010
MAT1-2-4core MAThit onlyDIAMOND, proteome41.2cov 97.6%6.4e-61185 bits277,841–278,668 -–746128_af293_MAT_MAT1-2ASPACDRAFT_9218
CIMG_00407core MAThit onlytblastn24.7cov 33.0%5.1e-0235 bits278,183–278,443 -–5501_rs_MAT_MAT1-2ASPACDRAFT_9218
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome36.5cov 41.1%1.1e-1156 bits279,215–280,281 +–230073_uamh-1363_MAT_MAT1-1ASPACDRAFT_1867751
MAT1-2-1core MAThit onlyDIAMOND, proteome61.8cov 25.4%2.1e-88261 bits279,215–280,281 +–1301501_voucher-267_MAT_MAT1-2ASPACDRAFT_1867751
APN2conserved flankhit onlyDIAMOND, proteome90.9cov 36.7%5.3e-305836 bits281,543–284,859 -–2903220_liq80xsp_MAT_combinedASPACDRAFT_117017
COX13conserved flankhit onlyDIAMOND, proteome78.8cov 100.0%3.1e-74211 bits285,291–286,159 +–746128_af293_MAT_MAT1-2ASPACDRAFT_42175

Withheld candidate loci 1

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (1).

Show the 1 withheld locus

Withheld loci (1)

familylocationidiomorph genesmodelledreason
MATKV878971:2,580,571–2,591,560MAT1-2MAT1-1-1, MAT1-2-1, MAT1-1-5, MAT1-2-101too few of the family's genes found

Provenance

Genome file
AaculeatusATCC16872.genome.fasta
Assembly
660 contigs · 35.4 Mb · N50 1.6 Mb
Genome SHA-256
78a9da3a9349077af3892ec4884e85b6343435001c7e2177f527fac46f503bdd
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:10:42Z
Run time
87 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits