MATPredict · mating-type report

PpennisetigenaBr36

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:00 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on RRCL01000008MAT1-1 locus: genes from left to right on RRCL01000008, 909,619 to 925,926: MAT1-1-1 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, minus strand).910 kb915 kb920 kb925 kb← 908.8 kb more to contig endcalled locusMAT1-1-1: core MAT gene, 909,619–910,699 (+), identity 35.1%, search hit onlyMAT1-1-1MAT1-2-1: core MAT gene, 912,634–913,885 (+), identity 37.9%, search hit onlyMAT1-2-1APN2: conserved flank, 914,581–916,949 (-), identity 86.4%, search hit onlyAPN2COX13: conserved flank, 924,839–925,926 (-), identity 68.3%, search hit onlyCOX13
Gene order on RRCL01000008. Scroll sideways to see the whole figure.
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
RRCL01000008:909,619–925,926 · 16.3 kb
Own genes
RRCL01000008:909,619–925,926 · 16.3 kb
Locus
Genes of both idiomorphs at one locus.
Genes modelled
4 of 4 gene hits
Nearest contig end
909.6 kb
Region (samtools)
RRCL01000008:909619-925926
Idiomorph scores
  • MAT1-1 97.4
  • MAT1-2 45.4

Margin over the next idiomorph: 52.0

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-1-1core MAThit onlyDIAMOND, proteome35.1cov 42.1%1.1e-2597 bits909,619–910,699 +–5127_mo44_MAT_MAT1-1PpBr36_09386
MAT1-2-1core MAThit onlyDIAMOND, proteome37.9cov 19.2%7.7e-0845 bits912,634–913,885 +–5016_c4_MAT_MAT1-2PpBr36_09388
APN2conserved flankhit onlyDIAMOND, proteome86.4cov 36.7%3.0e-240673 bits914,581–916,949 -–2903220_liq80xsp_MAT_combinedPpBr36_09389
COX13conserved flankhit onlyDIAMOND, proteome68.3cov 92.3%7.8e-59171 bits924,839–925,926 -–578113_sxlc146_MAT_MAT1-2PpBr36_09392
SLA2expected, not found–

Withheld candidate loci 3

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3).

Show the 3 withheld loci
familylocationidiomorph genesmodelledreason
MATCM014989:2,069,071–2,071,702MAT1-2MAT1-2-11too few of the family's genes found
MATCM014989:4,621,404–4,623,092MAT1-2MAT1-2-11too few of the family's genes found
MATRRCL01000008:801,587–805,019undeterminedSLA21too few of the family's genes found

Provenance

Genome file
PpennisetigenaBr36.genome.fasta
Assembly
108 contigs · 49.1 Mb · N50 5.4 Mb
Genome SHA-256
ad439cf6724f7eff186a72a058ea35d43b93f479f8d9e89160d1233230227b56
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 2528436, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 2528436', 'routed_by': 'family taxid 2528436 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:58:37Z
Run time
90 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits