MATPredict · mating-type report

MrestrictaKCTC27527

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:59 UTC by MATPredict 0.8.1

Result

Mating-type loci

undeterminedbLocushigh confidenceundeterminedaLocushigh confidence

Called loci 2

undetermined bLocus family · Basidiomycota

high confidence
Gene order of the undetermined locus on CP030254undetermined locus: genes from left to right on CP030254, 430,565 to 434,186: bE (core MAT gene, minus strand); bW (core MAT gene, plus strand).431 kb432 kb433 kb434 kb412.2 kb more to contig end →called locusbE: core MAT gene, 431,215–432,129 (-), identity 45.4%, search hit onlybEbW: core MAT gene, 432,606–434,186 (+), identity 77.1%, search hit onlybW
Gene order on CP030254. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on CP030254, 430,265 to 434,486MRET_2623 428,823-430,490 (-): uncharacterized proteinMRET_2623MRET_2624 430,565-431,119 (-): exosome complex protein LRP1MRET_2624MRET_2625 431,215-432,129 (-): homeobox domain proteinMRET_2625MRET_2626 432,606-434,186 (+): homeobox domain proteinMRET_2626MRET_2627 434,387-436,102 (+): methionine permeaseMRET_2627
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
CP030254:430,565–434,186 · 3.6 kb
Own genes
CP030254:431,215–434,186 · 3.0 kb · the span runs 650 bp beyond these genes
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
412.5 kb
Region (samtools)
CP030254:430565-434186

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
bEcore MAThit onlyDIAMOND, proteome45.4cov 97.6%2.7e-79235 bits431,215–432,129 -–76777_atcc-44340_bLocus_b2MRET_2625
bWcore MAThit onlyDIAMOND, proteome77.1cov 9.0%5.7e-75239 bits432,606–434,186 +–1882483_mca3645_bLocus_b1MRET_2626

undetermined aLocus family · Basidiomycota

high confidence
Gene order of the undetermined locus on CP030255undetermined locus: genes from left to right on CP030255, 606,874 to 608,743: pra1 (core MAT gene, minus strand); mfa1 (core MAT gene, plus strand).607.0 kb607.5 kb608.0 kb608.5 kb609.0 kb227.8 kb more to contig end →called locuspra1: core MAT gene, 606,874–608,069 (-), identity 31.8%, search hit onlypra1mfa1: core MAT gene, 608,594–608,743 (+), identity 46.0%, gene modelmfa1
Gene order on CP030255. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on CP030255, 606,574 to 609,043MRET_3186 605,660-606,745 (+): 26S proteasome regulatory subunit N10MRET_3186MRET_3187 606,874-608,069 (-): pheromone a factor receptorMRET_3187
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • gene model
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
CP030255:606,874–608,743 · 1.9 kb
Own genes
CP030255:606,874–608,743 · 1.9 kb
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
228.1 kb
Region (samtools)
CP030255:606874-608743

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
pra1core MAThit onlyDIAMOND, proteome31.8cov 81.3%6.4e-48159 bits606,874–608,069 -–120017_uh112_aLocus_a1MRET_3187
mfa1core MATgene modelexonerate46.0cov 100.0%1.0e-0847 bits608,594–608,743 +176777_unspecified-a1_aLocus_a1none
rba1expected, not found–

Provenance

Genome file
MrestrictaKCTC27527.genome.fasta
Assembly
10 contigs · 7.4 Mb · N50 1.2 Mb
Genome SHA-256
ebceaca824be18c07058ba1dd79f8c2aea1bbb6aaa232dda58def4f07b40f4c4
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'species', 'routing_taxid': 742845, 'routing_taxid_rank': 'family', 'flags': ['short_genome', 'identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 742845', 'routed_by': 'family taxid 742845 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T01:58:35Z
Run time
25 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits