MATPredict · mating-type report

MrestrictaCBS7877

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:58 UTC by MATPredict 0.8.1

Result

Mating-type loci

undeterminedbLocushigh confidenceundeterminedaLocushigh confidence

Called loci 2

undetermined bLocus family · Basidiomycota

high confidence
Gene order of the undetermined locus on CP033152undetermined locus: genes from left to right on CP033152, 360,779 to 364,421: bW (core MAT gene, minus strand); bE (core MAT gene, plus strand).361 kb362 kb363 kb364 kb← 360.5 kb more to contig endcalled locusbW: core MAT gene, 360,779–362,362 (-), identity 75.0%, search hit onlybWbE: core MAT gene, 362,854–363,765 (+), identity 45.6%, search hit onlybE
Gene order on CP033152. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on CP033152, 360,479 to 364,721DNF11_2869 358,863-360,578 (-): High-affinity methionine permeaseDNF11_2869DNF11_2870 360,779-362,362 (-): Homeobox-leucine zipper protein HOX20DNF11_2870DNF11_2871 362,854-363,765 (+): Mating-type locus allele B4 proteinDNF11_2871DNF11_2872 363,867-364,421 (+): Nuclear nucleic acid-binding protein C1DDNF11_2872DNF11_2873 364,495-366,162 (+): unspecified productDNF11_2873
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
CP033152:360,779–364,421 · 3.6 kb
Own genes
CP033152:360,779–363,765 · 3.0 kb · the span runs 656 bp beyond these genes
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
360.8 kb
Region (samtools)
CP033152:360779-364421

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
bWcore MAThit onlyDIAMOND, proteome75.0cov 9.0%5.8e-81255 bits360,779–362,362 -–1882483_mca3645_bLocus_b1DNF11_2870
bEcore MAThit onlyDIAMOND, proteome45.6cov 97.6%3.3e-80238 bits362,854–363,765 +–76777_atcc-42132_bLocus_b1DNF11_2871

undetermined aLocus family · Basidiomycota

high confidence
Gene order of the undetermined locus on CP033151undetermined locus: genes from left to right on CP033151, 606,067 to 607,706: pra1 (core MAT gene, minus strand); mfa1 (core MAT gene, plus strand).606.0 kb606.5 kb607.0 kb607.5 kb608.0 kb231.2 kb more to contig end →called locuspra1: core MAT gene, 606,334–607,021 (-), identity 24.07%, the two modelling tools disagreepra1mfa1: core MAT gene, 607,557–607,706 (+), identity 46.0%, gene modelmfa1
Gene order on CP033151. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on CP033151, 605,767 to 608,006DNF11_2529 605,829-606,468 (-): Pheromone receptor 1DNF11_2529
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • gene model
  • tools disagree
  • alternate model
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
CP033151:606,067–607,706 · 1.6 kb
Own genes
CP033151:606,334–607,706 · 1.4 kb · the span runs 267 bp beyond these genes
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
231.5 kb
Region (samtools)
CP033151:606067-607706

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
pra1core MATmodels differexonerateminiprot: 606,112–607,030, 23.8%24.1cov 57.1%1.3e-36107 bits606,334–607,021 -2203535_cbs-10006_aLocus_a1DNF11_2529
mfa1core MATgene modelexonerate46.0cov 100.0%4.8e-0947 bits607,557–607,706 +176777_unspecified-a1_aLocus_a1none
rba1expected, not found–

Withheld candidate loci 36

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (36).

Show the 36 withheld loci

Withheld loci (36)

familylocationidiomorph genesmodelledreason
aLocusCP033152:53,804–53,950undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033152:306,498–306,818undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033152:352,856–353,017undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033151:162,999–163,130undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033151:445,617–445,715undeterminedrba10fewer than 2 genes could be modelled
aLocusCP033151:531,045–531,224undeterminedrba10fewer than 2 genes could be modelled
aLocusCP033151:786,614–786,976undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:96,273–96,386undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:159,071–159,199undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:463,040–463,237undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:486,621–486,743undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:656,371–656,553undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:833,874–834,035undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:1,303,574–1,303,842undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033148:1,329,657–1,329,794undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033153:41,327–41,419undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033153:66,245–66,499undeterminedrba10fewer than 2 genes could be modelled
aLocusCP033153:160,617–160,781undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033153:407,675–407,767undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033153:452,163–452,279undeterminedrba10fewer than 2 genes could be modelled
aLocusCP033150:215,707–215,802undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033150:481,790–481,882undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033150:846,779–846,958undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033150:874,039–874,200undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033150:944,915–945,064undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033150:970,590–970,775undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033150:1,024,805–1,024,969undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033150:1,139,971–1,140,063undeterminedrba10fewer than 2 genes could be modelled
aLocusCP033149:60,199–60,291undeterminedrba10fewer than 2 genes could be modelled
aLocusCP033149:380,228–380,596undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033156:79,903–80,007undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033156:106,179–106,271undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033155:337,985–338,080undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033154:227,018–227,188undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033154:361,226–361,468undeterminedpra10fewer than 2 genes could be modelled
aLocusCP033154:446,886–447,083undeterminedpra10fewer than 2 genes could be modelled

Provenance

Genome file
MrestrictaCBS7877.genome.fasta
Assembly
10 contigs · 7.3 Mb · N50 1.2 Mb
Genome SHA-256
febda9fcc62d6a5cabb514eab43fe1bb8e46785a33b5ae16b0bd404bbaa2de3a
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'genus', 'routing_taxid': 742845, 'routing_taxid_rank': 'family', 'flags': ['short_genome'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 742845', 'routed_by': 'family taxid 742845 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T01:58:07Z
Run time
27 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits