MATPredict · mating-type report

CglobosumCBS148.51

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:02 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on CH408031MAT1-1 locus: genes from left to right on CH408031, 3,982,542 to 3,997,302: COX13 (conserved flank, minus strand); APN2 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-1-2 (core MAT gene, plus strand); MAT1-1-1 (core MAT gene, plus strand); SLA2 (conserved flank, minus strand).3,985 kb3,990 kb3,995 kb723.4 kb more to contig end →called locusCOX13: conserved flank, 3,982,542–3,983,363 (-), identity 63.5%, search hit onlyCOX13APN2: conserved flank, 3,984,073–3,986,398 (+), identity 84.1%, search hit onlyAPN2MAT1-1-3: core MAT gene, 3,987,784–3,988,623 (-), identity 46.9%, search hit onlyMAT1-1-3MAT1-2-1: core MAT gene, 3,988,065–3,988,166 (-), identity 50.0%, search hit onlyMAT1-2-1 (lost to MAT1-1-3)MAT1-1-2: core MAT gene, 3,989,174–3,990,529 (+), identity 33.5%, search hit onlyMAT1-1-2MAT1-1-1: core MAT gene, 3,990,896–3,991,905 (+), identity 57.1%, search hit onlyMAT1-1-1SLA2: conserved flank, 3,993,932–3,997,302 (-), identity 80.9%, search hit onlySLA2
Gene order on CH408031. Scroll sideways to see the whole figure.
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
CH408031:3,982,542–3,997,302 · 14.8 kb
Own genes
CH408031:3,982,542–3,997,302 · 14.8 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
6 of 7 gene hits
Nearest contig end
724.1 kb
Region (samtools)
CH408031:3982542-3997302
Idiomorph scores
  • MAT1-1 231.0

Margin over the set-aside MAT1-2-1 hit: -3.1 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-1-346.9MAT1-2-150.0100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
COX13conserved flankhit onlyDIAMOND, proteome63.5cov 100.0%2.2e-57167 bits3,982,542–3,983,363 -–27337_vdls-17_MAT_MAT1-2CHGG_06582
APN2conserved flankhit onlyDIAMOND, proteome84.1cov 36.7%1.8e-216610 bits3,984,073–3,986,398 +–2903220_liq80xsp_MAT_combinedCHGG_06583
MAT1-1-3core MAThit onlyDIAMOND, proteome46.9cov 19.8%1.4e-1463 bits3,987,784–3,988,623 -–5141_74-ors-a_MAT_MAT1-1CHGG_06584
MAT1-2-1core MATlost to MAT1-1-3, not countedhit onlytblastn50.0cov 8.0%8.8e-0940 bits3,988,065–3,988,166 -–1873960_unknown-2_MAT_MAT1-2CHGG_06584
MAT1-1-2core MAThit onlyDIAMOND, proteome33.5cov 51.5%1.3e-31115 bits3,989,174–3,990,529 +–5141_74-ors-a_MAT_MAT1-1CHGG_06585
MAT1-1-1core MAThit onlyDIAMOND, proteome57.1cov 66.9%1.3e-77231 bits3,990,896–3,991,905 +–5141_74-ors-a_MAT_MAT1-1CHGG_06586
SLA2conserved flankhit onlyDIAMOND, proteome80.9cov 100.0%< 1e-1801,436 bits3,993,932–3,997,302 -–107463_unknown-1_MAT_MAT1-2CHGG_06587

Withheld candidate loci 4

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (4).

Show the 4 withheld loci
familylocationidiomorph genesmodelledreason
MATCH408030:1,949,207–1,950,956MAT1-2MAT1-2-11too few of the family's genes found
MATCH408030:4,650,124–4,667,645MAT1-2MAT1-1-1, MAT1-2-1, MAT1-1-22too few of the family's genes found
MATCH408030:4,837,129–4,841,531MAT1-2MAT1-2-11too few of the family's genes found
MATCH408033:4,153,169–4,155,277MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
CglobosumCBS148.51.genome.fasta
Assembly
37 contigs · 34.9 Mb · N50 4.7 Mb
Genome SHA-256
88ed49f126c10f93ff2c7e4ce0450050d5cdc71a8e8633449f06a27b8836ab0e
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 35718, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 35718', 'routed_by': 'family taxid 35718 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:01:08Z
Run time
98 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits