MATPredict · mating-type report

Smacrosporak-hell

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:42 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1high confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on CABT02000006MAT1-1 locus: genes from left to right on CABT02000006, 1,151,337 to 1,169,685: SLA2 (conserved flank, plus strand); MAT1-1-1 (core MAT gene, minus strand); MAT1-1-2 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).1,155 kb1,160 kb1,165 kb1,170 kb267.0 kb more to contig end →called locusSLA2: conserved flank, 1,151,337–1,154,650 (+), identity 80.2%, search hit onlySLA2MAT1-1-1: core MAT gene, 1,158,372–1,159,411 (-), identity 70.9%, search hit onlyMAT1-1-1MAT1-1-2: core MAT gene, 1,159,818–1,161,128 (-), identity 71.8%, search hit onlyMAT1-1-2MAT1-1-3: core MAT gene, 1,161,860–1,162,425 (+), identity 76.3%, search hit onlyMAT1-1-3MAT1-2-1: core MAT gene, 1,163,074–1,164,047 (+), identity 86.0%, search hit onlyMAT1-2-1APN2: conserved flank, 1,165,832–1,168,030 (-), identity 84.1%, search hit onlyAPN2COX13: conserved flank, 1,168,777–1,169,685 (+), identity 72.6%, search hit onlyCOX13
Gene order on CABT02000006. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on CABT02000006, 1,150,421 to 1,170,603SMAC_05400 1,151,337-1,154,650 (+): I/LWEQ domain | ANTH domain | domain-containing proteinSMAC_05400SMAC_05401 1,158,372-1,159,411 (-): SmtA1SMAC_05401 SmtA1SMAC_05402 1,159,818-1,161,128 (-): SmtA2SMAC_05402 SmtA2SMAC_05403 1,161,860-1,162,425 (+): SmtA3SMAC_05403 SmtA3SMAC_05404 1,163,074-1,164,047 (+): Smta1SMAC_05404 SmtA1SMAC_05405 1,165,832-1,168,030 (-): DNA-(apurinic or apyrimidinic site) endonuclease 2SMAC_05405SMAC_05406 1,168,777-1,169,685 (+): Cytochrome c oxidase subunit VIa | domain-containing proteinSMAC_05406SMAC_05407 1,170,309-1,172,867 (-): putative apc5SMAC_05407 putative apc5
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
CABT02000006:1,151,337–1,169,685 · 18.3 kb
Own genes
CABT02000006:1,151,337–1,169,685 · 18.3 kb
Locus
Genes of both idiomorphs at one locus.
Genes modelled
7 of 7 gene hits
Nearest contig end
267.9 kb
Region (samtools)
CABT02000006:1151337-1169685
Idiomorph scores
  • MAT1-1 523.0
  • MAT1-2 511.0

Margin over the next idiomorph: 12.0

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-186.0MAT1-1-340.0100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome80.2cov 100.0%< 1e-1801,355 bits1,151,337–1,154,650 +–230073_uamh-1363_MAT_MAT1-1SMAC_05400
MAT1-1-1core MAThit onlyDIAMOND, proteome70.9cov 100.0%2.4e-160442 bits1,158,372–1,159,411 -–5141_74-ors-a_MAT_MAT1-1SMAC_05401 SmtA1
MAT1-1-2core MAThit onlyDIAMOND, proteome71.8cov 96.0%2.7e-190523 bits1,159,818–1,161,128 -–5141_74-ors-a_MAT_MAT1-1SMAC_05402 SmtA2
MAT1-1-3core MAThit onlyDIAMOND, proteome76.3cov 29.9%3.2e-50155 bits1,161,860–1,162,425 +–5141_74-ors-a_MAT_MAT1-1SMAC_05403 SmtA3
MAT1-2-1core MAThit onlyDIAMOND, proteome86.0cov 73.8%1.1e-186511 bits1,163,074–1,164,047 +–5141_unknown-1_MAT_MAT1-2SMAC_05404 SmtA1
APN2conserved flankhit onlyDIAMOND, proteome84.1cov 36.7%6.7e-227638 bits1,165,832–1,168,030 -–2903220_liq80xsp_MAT_combinedSMAC_05405
COX13conserved flankhit onlyDIAMOND, proteome72.6cov 67.9%1.2e-51153 bits1,168,777–1,169,685 +–199306_rmscc1040_MAT_MAT1-1SMAC_05406

Withheld candidate loci 3

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3).

Show the 3 withheld loci

Withheld loci (3)

familylocationidiomorph genesmodelledreason
MATCABT02000007:1,044,200–1,045,579undeterminedAPN21too few of the family's genes found
MATCABT02000019:120,274–123,471MAT1-2MAT1-2-11too few of the family's genes found
MATCABT02000042:11,748–16,416MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
Smacrosporak-hell.genome.fasta
Assembly
1,212 contigs · 38.2 Mb · N50 745.8 kb
Genome SHA-256
37e503ceb53affea38b99b2e431187569e3f3e5a1d5720c287d804dee65b084f
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 5148, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 5148', 'routed_by': 'family taxid 5148 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:40:49Z
Run time
106 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits