MATPredict · mating-type report

PzonataCBS506.65

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:46 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2high confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on KV878352MAT1-2 locus: genes from left to right on KV878352, 223,605 to 236,471: SLA2 (conserved flank, plus strand); MAT1-2-4 (core MAT gene, minus strand); CIMG_00407 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).225 kb230 kb235 kb203.9 kb more to contig end →called locusSLA2: conserved flank, 223,605–227,546 (+), identity 88.2%, search hit onlySLA2MAT1-2-4: core MAT gene, 229,808–230,646 (-), identity 46.2%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 230,233–230,508 (-), identity 25.0%, search hit onlyCIMG_00407MAT1-1-3: core MAT gene, 231,245–232,332 (+), identity 35.5%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 231,245–232,332 (+), identity 58.8%, search hit onlyMAT1-2-1APN2: conserved flank, 233,182–235,366 (-), identity 88.6%, search hit onlyAPN2COX13: conserved flank, 235,718–236,471 (+), identity 72.1%, search hit onlyCOX13
Gene order on KV878352. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KV878352, 222,962 to 237,113ASPZODRAFT_136258 219,931-223,252 (+): Pleckstrin homology domain | domain-containing proteinASPZODRAFT_136258ASPZODRAFT_136259 223,359-227,561 (+): I/LWEQ domain | ANTH domain | domain-containing proteinASPZODRAFT_136259ASPZODRAFT_136260 229,310-230,659 (-): hypothetical proteinASPZODRAFT_136260ASPZODRAFT_146122 231,245-232,332 (+): HMG (high mobility group) box | domain-containing proteinASPZODRAFT_146122ASPZODRAFT_161632 233,086-235,498 (-): DNA-(apurinic or apyrimidinic site) endonuclease 2ASPZODRAFT_161632ASPZODRAFT_123649 235,676-236,639 (+): Cytochrome c oxidase subunit VIa | domain-containing proteinASPZODRAFT_123649ASPZODRAFT_136263 236,838-239,852 (-): Anaphase-promoting complex subunit 5 | domain-containing proteinASPZODRAFT_136263
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KV878352:223,605–236,471 · 12.9 kb
Own genes
KV878352:223,605–236,471 · 12.9 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
204.5 kb
Region (samtools)
KV878352:223605-236471
Idiomorph scores
  • MAT1-2 286.0

Margin over the set-aside MAT1-1-3 hit: 23.3 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-158.8MAT1-1-335.5100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome88.2cov 100.0%< 1e-1801,590 bits223,605–227,546 +–107463_unknown-1_MAT_MAT1-2ASPZODRAFT_136259
MAT1-2-4core MAThit onlyDIAMOND, proteome46.2cov 87.2%2.8e-63191 bits229,808–230,646 -–746128_af293_MAT_MAT1-2ASPZODRAFT_136260
CIMG_00407core MAThit onlytblastn25.0cov 34.0%2.0e-0339 bits230,233–230,508 -–5501_rs_MAT_MAT1-2ASPZODRAFT_136260
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome35.5cov 50.6%3.5e-1257 bits231,245–232,332 +–230073_uamh-1363_MAT_MAT1-1ASPZODRAFT_146122
MAT1-2-1core MAThit onlyDIAMOND, proteome58.8cov 27.1%3.2e-98286 bits231,245–232,332 +–1301501_voucher-267_MAT_MAT1-2ASPZODRAFT_146122
APN2conserved flankhit onlyDIAMOND, proteome88.6cov 36.7%2.7e-296814 bits233,182–235,366 -–2903220_liq80xsp_MAT_combinedASPZODRAFT_161632
COX13conserved flankhit onlyDIAMOND, proteome72.1cov 100.0%9.4e-68194 bits235,718–236,471 +–746128_af293_MAT_MAT1-2ASPZODRAFT_123649

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATKV878338:1,560,132–1,589,320MAT1-1MAT1-1-1, MAT1-1-31too few of the family's genes found
MATKV878349:245,422–268,005MAT1-2MAT1-1-1, MAT1-2-11too few of the family's genes found

Provenance

Genome file
PzonataCBS506.65.genome.fasta
Assembly
246 contigs · 26.1 Mb · N50 1.2 Mb
Genome SHA-256
4ab75b952b483461267570be217b5807c733e67d3e3bb53f55f52952403b7ff9
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:45:47Z
Run time
57 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits