MATPredict · mating-type report

MpoaeATCC64411

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:36 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1medium confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-1 locus on GL876978MAT1-1 locus: genes from left to right on GL876978, 193,941 to 207,113: SLA2 (conserved flank, plus strand); MAT1-1-1 (core MAT gene, plus strand); MAT1-1-2 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand).195 kb200 kb205 kb← 193.3 kb more to contig endcalled locusSLA2: conserved flank, 193,941–197,034 (+), identity 97.6%, search hit onlySLA2MAT1-1-1: core MAT gene, 199,155–200,049 (+), identity 40.0%, search hit onlyMAT1-1-1MAT1-1-2: core MAT gene, 201,111–201,281 (-), identity 35.593%, search hit onlyMAT1-1-2MAT1-2-1: core MAT gene, 202,737–202,850 (+), identity 34.211%, search hit onlyMAT1-2-1APN2: conserved flank, 204,710–207,113 (-), identity 84.1%, search hit onlyAPN2
Gene order on GL876978. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on GL876978, 193,284 to 207,771MAPG_10729 192,453-197,034 (+): cytoskeleton assembly control protein Sla2MAPG_10729MAPG_10730 197,479-200,509 (+): Mating-type protein MAT alpha 1 HMG-box | domain-containing proteinMAPG_10730MAPG_10731 200,500-201,909 (-): Mating type protein 1-1-2 of unknown function | domain-containing proteinMAPG_10731MAPG_10732 202,233-203,877 (+): HMG (high mobility group) box | domain-containing proteinMAPG_10732MAPG_10733 204,710-207,113 (-): DNA-(apurinic or apyrimidinic site) lyase 2MAPG_10733
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
GL876978:193,941–207,113 · 13.2 kb
Own genes
GL876978:193,941–207,113 · 13.2 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
3 of 5 gene hits
Nearest contig end
193.9 kb
Region (samtools)
GL876978:193941-207113
Idiomorph scores
  • MAT1-1 100.0
  • MAT1-2 29.3

Margin over the next idiomorph: 70.7

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome97.6cov 27.6%< 1e-1801,240 bits193,941–197,034 +–107463_unknown-1_MAT_MAT1-2MAPG_10729
MAT1-1-1core MAThit onlyDIAMOND, proteome40.0cov 26.6%2.5e-27100 bits199,155–200,049 +–1301501_voucher-215_MAT_MAT1-1MAPG_10730
MAT1-1-2core MAThit onlytblastn35.6cov 17.0%2.0e-0340 bits201,111–201,281 -–230073_uamh-1363_MAT_MAT1-1MAPG_10731
MAT1-2-1core MAThit onlytblastn34.2cov 11.0%6.1e+0029 bits202,737–202,850 +–13684_sn436ga98_MAT_MAT1-2MAPG_10732
APN2conserved flankhit onlyDIAMOND, proteome84.1cov 36.7%8.9e-222628 bits204,710–207,113 -–2903220_liq80xsp_MAT_combinedMAPG_10733
COX13expected, not found–

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATGL876973:512,641–513,914MAT1-2MAT1-2-11too few of the family's genes found
MATGL877104:62–2,008MAT1-1COX13, MAT1-1-31too few of the family's genes found

Provenance

Genome file
MpoaeATCC64411.genome.fasta
Assembly
206 contigs · 39.6 Mb · N50 3.4 Mb
Genome SHA-256
1ceecbfe337de756f106928e08cf7f3f3f4cddad4e2700888bd8d3d21b2a57dc
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 81093, 'routing_taxid_rank': 'family', 'flags': ['high_N', 'identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 81093', 'routed_by': 'family taxid 81093 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:35:21Z
Run time
68 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits