MATPredict · mating-type report

Mmaydis521

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:32 UTC by MATPredict 0.8.1

Result

Mating-type loci

undeterminedbLocushigh confidenceundeterminedaLocushigh confidence

Called loci 2

undetermined bLocus family · Basidiomycota

high confidence
Gene order of the undetermined locus on CM003140undetermined locus: genes from left to right on CM003140, 1,688,370 to 1,693,905: bE (core MAT gene, minus strand); bW (core MAT gene, plus strand).1,689 kb1,690 kb1,691 kb1,692 kb1,693 kb1,694 kb782.3 kb more to contig end →called locusbE: core MAT gene, 1,690,004–1,691,499 (-), identity 100.0%, search hit onlybEbW: core MAT gene, 1,691,754–1,693,905 (+), identity 100.0%, search hit onlybW
Gene order on CM003140. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on CM003140, 1,688,070 to 1,694,205UMAG_00575 1,685,553-1,688,201 (+): unspecified productUMAG_00575UMAG_00576 1,688,370-1,689,083 (-): Sas10/Utp3/C1D family | domain-containing proteinUMAG_00576UMAG_12052 1,690,004-1,691,499 (-): b mating type locus, bE1 alleleUMAG_12052UMAG_00578 1,691,754-1,693,905 (+): b mating type locus, bW1 alleleUMAG_00578
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
CM003140:1,688,370–1,693,905 · 5.5 kb
Own genes
CM003140:1,690,004–1,693,905 · 3.9 kb · the span runs 1.6 kb beyond these genes
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
782.6 kb
Region (samtools)
CM003140:1688370-1693905

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
bEcore MAThit onlyDIAMOND, proteome100.0cov 100.0%< 1e-180916 bits1,690,004–1,691,499 -–5270_521_bLocus_b1UMAG_12052
bWcore MAThit onlyDIAMOND, proteome100.0cov 100.0%< 1e-1801,206 bits1,691,754–1,693,905 +–5270_521_bLocus_b1UMAG_00578

undetermined aLocus family · Basidiomycota

high confidence
Gene order of the undetermined locus on CM003144undetermined locus: genes from left to right on CM003144, 1,023,522 to 1,028,134: mfa1 (core MAT gene, minus strand); pra1 (core MAT gene, minus strand); rba1 (variable flank, minus strand).1,024 kb1,025 kb1,026 kb1,027 kb1,028 kb365.0 kb more to contig end →called locusmfa1: core MAT gene, 1,023,522–1,023,644 (-), identity 100.0%, search hit onlymfa1pra1: core MAT gene, 1,025,469–1,026,779 (-), identity 100.0%, search hit onlypra1rba1: variable flank, 1,027,492–1,028,134 (-), identity 100.0%, search hit onlyrba1
Gene order on CM003144. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on CM003144, 1,023,222 to 1,028,434UMAG_02382 1,023,522-1,023,644 (-): a1-specific pheromoneUMAG_02382 MFA1UMAG_02383 1,025,469-1,026,779 (-): a2-pheromone receptor Pra1UMAG_02383 PRA1UMAG_02384 1,027,492-1,028,134 (-): a1-mating type protein Rba1UMAG_02384UMAG_10139 1,028,379-1,029,680 (-): putative pantoate--beta-alanine ligaseUMAG_10139
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • variable flank
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
CM003144:1,023,522–1,028,134 · 4.6 kb
Own genes
CM003144:1,023,522–1,028,134 · 4.6 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
3 of 3 gene hits
Nearest contig end
365.3 kb
Region (samtools)
CM003144:1023522-1028134

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
mfa1core MAThit onlyDIAMOND, proteome100.0cov 100.0%1.0e-2478 bits1,023,522–1,023,644 -–5270_521_aLocus_a1UMAG_02382 MFA1
pra1core MAThit onlyDIAMOND, proteome100.0cov 100.0%1.5e-258694 bits1,025,469–1,026,779 -–5270_521_aLocus_a1UMAG_02383 PRA1
rba1variable flankhit onlyDIAMOND, proteome100.0cov 100.0%3.0e-86239 bits1,027,492–1,028,134 -–5270_521_aLocus_a1UMAG_02384

Provenance

Genome file
Mmaydis521.genome.fasta
Assembly
27 contigs · 19.7 Mb · N50 885.0 kb
Genome SHA-256
0d5a2562baec11460f9937938b94aebfc066a4b6d6d92e50fca30363208e93a1
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'species', 'routing_taxid': 5267, 'routing_taxid_rank': 'order', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'order taxid 5267', 'routed_by': 'order taxid 5267 (direct)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:32:33Z
Run time
8 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits