MATPredict · mating-type report

Mlarici-populina98AG31

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:31 UTC by MATPredict 0.8.1

Result

Mating type

undeterminedhigh confidence

Called loci 1

undetermined rustHD family · Basidiomycota

high confidence
Gene order of the undetermined locus on GL883124undetermined locus: genes from left to right on GL883124, 688,203 to 691,501: bW (core MAT gene, minus strand); bE (core MAT gene, plus strand).688 kb689 kb690 kb691 kb305.7 kb more to contig end →called locusbW: core MAT gene, 688,203–689,635 (-), identity 100.0%, search hit onlybWbE: core MAT gene, 690,174–691,501 (+), identity 100.0%, search hit onlybE
Gene order on GL883124. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on GL883124, 687,903 to 691,801MELLADRAFT_90168 688,203-689,635 (-): TALE-type homeodomain proteinMELLADRAFT_90168 MplbE1MELLADRAFT_124184 690,075-691,501 (+): putative b mating type locusMELLADRAFT_124184 MlpbW1
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
GL883124:688,203–691,501 · 3.3 kb
Own genes
GL883124:688,203–691,501 · 3.3 kb
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
306.0 kb
Region (samtools)
GL883124:688203-691501

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
bWcore MAThit onlyDIAMOND, proteome100.0cov 100.0%7.4e-280754 bits688,203–689,635 -–747676_98ag31_rustHD_b1MELLADRAFT_90168 MplbE1
bEcore MAThit onlyDIAMOND, proteome100.0cov 100.0%6.9e-268719 bits690,174–691,501 +–747676_98ag31_rustHD_b1MELLADRAFT_124184 MlpbW1

Provenance

Genome file
Mlarici-populina98AG31.genome.fasta
Assembly
462 contigs · 101.1 Mb · N50 1.1 Mb
Genome SHA-256
40db5ecc03dde8b71e7c7b313a10d8e9b283ffc5e12275a97f5ff8585589106a
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'species', 'routing_taxid': 5259, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 5259', 'routed_by': 'family taxid 5259 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:31:45Z
Run time
10 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits