MATPredict · mating-type report

FsolaniFSSC5MPISDFRAT0091

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:49 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1high confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on JAGTJS010000004MAT1-1 locus: genes from left to right on JAGTJS010000004, 2,809,300 to 2,827,163: COX13 (conserved flank, minus strand); APN2 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-1-2 (core MAT gene, plus strand); MAT1-1-1 (core MAT gene, plus strand); SLA2 (conserved flank, minus strand).2,810 kb2,815 kb2,820 kb2,825 kb334.2 kb more to contig end →called locusCOX13: conserved flank, 2,809,300–2,810,061 (-), identity 68.2%, search hit onlyCOX13APN2: conserved flank, 2,810,589–2,813,026 (+), identity 86.4%, search hit onlyAPN2MAT1-1-3: core MAT gene, 2,813,974–2,814,757 (-), identity 37.6%, search hit onlyMAT1-1-3MAT1-2-1: core MAT gene, 2,814,061–2,814,261 (-), identity 32.836%, search hit onlyMAT1-2-1 (lost to MAT1-1-3)MAT1-1-2: core MAT gene, 2,815,112–2,816,612 (+), identity 32.8%, search hit onlyMAT1-1-2MAT1-1-1: core MAT gene, 2,817,081–2,818,255 (+), identity 41.7%, search hit onlyMAT1-1-1SLA2: conserved flank, 2,823,850–2,827,163 (-), identity 80.1%, search hit onlySLA2
Gene order on JAGTJS010000004. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on JAGTJS010000004, 2,808,407 to 2,828,056B0J15DRAFT_440946 2,805,972-2,808,934 (-): anaphase-promoting complex subunit 5-domain-containing proteinB0J15DRAFT_440946B0J15DRAFT_485991 2,809,191-2,810,114 (-): cytochrome c oxidase, subunit VIaB0J15DRAFT_485991B0J15DRAFT_485992 2,810,280-2,813,513 (+): Endonuclease/exonuclease/phosphataseB0J15DRAFT_485992B0J15DRAFT_544689 2,813,974-2,814,757 (-): HMG (high mobility group) box | domain-containing proteinB0J15DRAFT_544689B0J15DRAFT_522728 2,815,112-2,816,709 (+): Mating type protein 1-1-2 of unknown function | domain-containing proteinB0J15DRAFT_522728B0J15DRAFT_418265 2,817,016-2,818,391 (+): mating-type protein MAT alpha 1-domain-containing proteinB0J15DRAFT_418265B0J15DRAFT_461833 2,819,788-2,820,189 (-): XM_046273648.1B0J15DRAFT_461833B0J15DRAFT_544692 2,820,970-2,822,319 (+): XM_046280940.1B0J15DRAFT_544692B0J15DRAFT_224429 2,823,759-2,827,258 (-): ANTH domain-containing proteinB0J15DRAFT_224429
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
JAGTJS010000004:2,809,300–2,827,163 · 17.9 kb
Own genes
JAGTJS010000004:2,809,300–2,827,163 · 17.9 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
6 of 7 gene hits
Nearest contig end
335.1 kb
Region (samtools)
JAGTJS010000004:2809300-2827163
Idiomorph scores
  • MAT1-1 212.0

Margin over the set-aside MAT1-2-1 hit: 4.8 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-1-141.7MAT1-2-126.7100.0higher identity
MAT1-1-337.6MAT1-2-132.8100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
COX13conserved flankhit onlyDIAMOND, proteome68.2cov 96.9%1.4e-64186 bits2,809,300–2,810,061 -–578113_sxlc146_MAT_MAT1-2B0J15DRAFT_485991
APN2conserved flankhit onlyDIAMOND, proteome86.4cov 36.7%7.6e-221617 bits2,810,589–2,813,026 +–2903220_liq80xsp_MAT_combinedB0J15DRAFT_485992
MAT1-1-3core MAThit onlyDIAMOND, proteome37.6cov 71.0%2.1e-37122 bits2,813,974–2,814,757 -–5127_mo44_MAT_MAT1-1B0J15DRAFT_544689
MAT1-2-1core MATlost to MAT1-1-3, not countedhit onlytblastn32.8cov 40.0%3.9e-0439 bits2,814,061–2,814,261 -–61372_suf959_MAT_MAT1-2B0J15DRAFT_544689
MAT1-1-2core MAThit onlyDIAMOND, proteome32.8cov 86.6%2.6e-66212 bits2,815,112–2,816,612 +–5518_3639_MAT_combinedB0J15DRAFT_522728
MAT1-1-1core MAThit onlyDIAMOND, proteome41.7cov 16.9%2.4e-64201 bits2,817,081–2,818,255 +–230073_uamh-1363_MAT_MAT1-1B0J15DRAFT_418265
SLA2conserved flankhit onlyDIAMOND, proteome80.1cov 97.0%< 1e-1801,367 bits2,823,850–2,827,163 -–5158_cbs114723_MAT_unswitchedB0J15DRAFT_224429

Withheld candidate loci 3

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3).

Show the 3 withheld loci

Withheld loci (3)

familylocationidiomorph genesmodelledreason
MATJAGTJS010000002:4,245,541–4,247,156MAT1-2MAT1-2-11too few of the family's genes found
MATJAGTJS010000003:653,966–656,095MAT1-2MAT1-2-11too few of the family's genes found
MATJAGTJS010000042:22,087–22,614MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
FsolaniFSSC5MPISDFRAT0091.genome.fasta
Assembly
74 contigs · 52.9 Mb · N50 2.2 Mb
Genome SHA-256
8c7fd275bcedb354b7ea44ef0b4ebe15def65d02d65f28155dd9488311c85d5e
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 110618, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 110618', 'routed_by': 'family taxid 110618 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:47:26Z
Run time
112 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits