MATPredict · mating-type report

CduobushaemulonisB09383

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:17 UTC by MATPredict 0.8.1

Result

Mating type

alphahigh confidence

Called loci 1

alpha MTL family · Ascomycota

high confidence
Gene order of the alpha locus on PKFP01000003alpha locus: genes from left to right on PKFP01000003, 1,474,613 to 1,483,311: OBP1 (variable flank, plus strand); PIK1 (variable flank, plus strand); MTLalpha1 (core MAT gene, plus strand); PAP1 (variable flank, minus strand).1,476 kb1,478 kb1,480 kb1,482 kb175.1 kb more to contig end →called locusOBP1: variable flank, 1,474,613–1,475,803 (+), identity 84.4%, search hit onlyOBP1PIK1: variable flank, 1,476,030–1,478,828 (+), identity 79.7%, search hit onlyPIK1MTLalpha1: core MAT gene, 1,478,982–1,479,416 (+), identity 56.55%, gene modelMTLalpha1PAP1: variable flank, 1,479,443–1,483,311 (-), identity 87.9%, search hit onlyPAP1
Gene order on PKFP01000003. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on PKFP01000003, 1,474,179 to 1,483,745CXQ87_003537 1,473,976-1,474,278 (-): hypothetical proteinCXQ87_003537CXQ87_003538 1,474,613-1,475,803 (+): Oxysterol-binding protein | domain-containing proteinCXQ87_003538CXQ87_003539 1,476,030-1,478,828 (+): 1-phosphatidylinositol 4-kinaseCXQ87_003539CXQ87_003540 1,479,443-1,483,311 (-): Poly(A) polymerase PAPalphaCXQ87_003540
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • variable flank
  • gene model
  • search hit only
Location
PKFP01000003:1,474,613–1,483,311 · 8.7 kb
Cross-lineage class
MAT1-1
Own genes
PKFP01000003:1,474,613–1,483,311 · 8.7 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
4 of 4 gene hits
Nearest contig end
175.5 kb
Region (samtools)
PKFP01000003:1474613-1483311
Idiomorph scores
  • alpha 174.0

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
OBP1variable flankhit onlyDIAMOND, proteome84.4cov 100.0%1.4e-251680 bits1,474,613–1,475,803 +–498019_b11221_MTL_alphaCXQ87_003538
PIK1variable flankhit onlyDIAMOND, proteome79.7cov 100.0%< 1e-1801,391 bits1,476,030–1,478,828 +–498019_b11221_MTL_alphaCXQ87_003539
MTLalpha1core MATgene modelexonerate56.5cov 84.3%3.1e-51174 bits1,478,982–1,479,416 +1498019_b11221_MTL_alphanone
PAP1variable flankhit onlyDIAMOND, proteome87.9cov 100.0%< 1e-180998 bits1,479,443–1,483,311 -–498019_b11221_MTL_alphaCXQ87_003540

Withheld candidate loci 44

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (41); too few of the family's genes found (3).

Show the 44 withheld loci

Withheld loci (44)

familylocationidiomorph genesmodelledreason
MTLPKFP01000001:480,508–480,720alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000001:605,543–605,674alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000001:824,200–824,298AMTLA20fewer than 2 genes could be modelled
MTLPKFP01000001:1,038,267–1,038,575AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000001:1,217,651–1,241,982AMTLA1, PIK11fewer than 2 genes could be modelled
MTLPKFP01000001:1,353,856–1,354,023alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000001:1,398,016–1,398,210alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000001:2,754,468–2,754,569AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000001:3,117,292–3,117,408alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000001:3,162,109–3,162,402AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000002:338,243–338,434alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000003:153,700–154,212alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000003:440,732–440,971alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000003:637,463–637,774AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000003:1,004,265–1,004,363alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000003:1,311,527–1,311,619AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000005:225,158–225,337AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000005:250,904–251,002alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000005:347,596–347,700alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000005:466,749–466,913alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000004:94,159–94,296alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000004:747,157–747,321alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000004:1,174,090–1,174,278alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000004:1,349,064–1,349,222AMTLA20fewer than 2 genes could be modelled
MTLPKFP01000004:1,482,388–1,482,510AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000004:1,551,748–1,551,963AMTLA20fewer than 2 genes could be modelled
MTLPKFP01000008:18,261–18,350AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000008:851,722–851,832alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000008:954,898–955,122alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000008:989,520–989,684alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000008:1,493,720–1,493,812AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000008:1,807,879–1,807,995AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000008:2,538,330–2,538,491AMTLA20fewer than 2 genes could be modelled
MTLPKFP01000008:2,677,886–2,677,984alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000008:2,746,924–2,747,055alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000008:2,814,240–2,814,362alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000008:3,088,378–3,088,557AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000008:3,173,377–3,173,484AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000006:23,527–23,787alphaMTLalpha10fewer than 2 genes could be modelled
MTLPKFP01000006:783,531–783,641AMTLA10fewer than 2 genes could be modelled
MTLPKFP01000006:1,013,029–1,013,124AMTLA20fewer than 2 genes could be modelled
MTLPKFP01000001:1,464,332–1,465,591undeterminedOBP11too few of the family's genes found
MTLPKFP01000001:2,350,266–2,356,019undeterminedPIK11too few of the family's genes found
MTLPKFP01000002:453,195–455,753undeterminedOBP11too few of the family's genes found

Provenance

Genome file
CduobushaemulonisB09383.genome.fasta
Assembly
8 contigs · 12.6 Mb · N50 3.3 Mb
Genome SHA-256
c4cf1e760aee1d5bc70b98bb4e49a4ddb840c0a8bdbeb3e949684027864caa48
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 27319, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 12, 'genetic_code_from': 'family taxid 27319', 'routed_by': 'family taxid 27319 (lineage)'}, genetic_code=12, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:16:52Z
Run time
21 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits