MATPredict · mating-type report

AsteyniiIBT23096

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:32 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2medium confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on MSFO01000002MAT1-2 locus: genes from left to right on MSFO01000002, 860,587 to 873,738: COX13 (conserved flank, minus strand); APN2 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-2-4 (core MAT gene, plus strand); CIMG_00407 (core MAT gene, plus strand); SLA2 (conserved flank, minus strand).860 kb865 kb870 kb← 859.9 kb more to contig endcalled locusCOX13: conserved flank, 860,587–861,366 (-), identity 74.5%, search hit onlyCOX13APN2: conserved flank, 861,691–863,717 (+), identity 90.9%, search hit onlyAPN2MAT1-1-3: core MAT gene, 864,704–865,711 (-), identity 31.6%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 864,704–865,711 (-), identity 58.3%, search hit onlyMAT1-2-1MAT1-2-4: core MAT gene, 866,222–867,123 (+), identity 45.5%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 866,444–866,710 (+), identity 27.368%, search hit onlyCIMG_00407SLA2: conserved flank, 869,849–873,738 (-), identity 87.5%, search hit onlySLA2
Gene order on MSFO01000002. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on MSFO01000002, 859,930 to 874,396P170DRAFT_453296 857,523-860,381 (+): anaphase-promoting complex proteinP170DRAFT_453296P170DRAFT_461837 860,490-861,435 (-): putative cytochrome c oxidase subunit VIaP170DRAFT_461837P170DRAFT_461838 861,602-864,112 (+): DNA lyase Apn2P170DRAFT_461838P170DRAFT_349471 864,704-865,711 (-): mating-type HMG-box protein MAT1-2P170DRAFT_349471P170DRAFT_422662 866,222-867,123 (+): putative mating locus proteinP170DRAFT_422662P170DRAFT_82848 867,029-867,477 (+): hypothetical proteinP170DRAFT_82848P170DRAFT_82841 867,342-868,179 (-): hypothetical proteinP170DRAFT_82841P170DRAFT_376660 869,532-873,952 (-): endocytic adaptor proteinP170DRAFT_376660P170DRAFT_433552 874,268-877,558 (-): Pleckstrin homology domain | domain-containing proteinP170DRAFT_433552
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
MSFO01000002:860,587–873,738 · 13.2 kb
Own genes
MSFO01000002:860,587–873,738 · 13.2 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
860.6 kb
Region (samtools)
MSFO01000002:860587-873738
Idiomorph scores
  • MAT1-2 333.0

Margin over the set-aside MAT1-1-3 hit: 26.7 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-158.3MAT1-1-331.6100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
COX13conserved flankhit onlyDIAMOND, proteome74.5cov 100.0%2.9e-71203 bits860,587–861,366 -–746128_af293_MAT_MAT1-2P170DRAFT_461837
APN2conserved flankhit onlyDIAMOND, proteome90.9cov 36.7%1.2e-294808 bits861,691–863,717 +–2903220_liq80xsp_MAT_combinedP170DRAFT_461838
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome31.6cov 42.2%1.5e-1258 bits864,704–865,711 -–230073_uamh-1363_MAT_MAT1-1P170DRAFT_349471
MAT1-2-1core MAThit onlyDIAMOND, proteome58.3cov 99.4%5.4e-117333 bits864,704–865,711 -–746128_af293_MAT_MAT1-2P170DRAFT_349471
MAT1-2-4core MAThit onlyDIAMOND, proteome45.5cov 96.8%8.5e-76223 bits866,222–867,123 +–746128_af293_MAT_MAT1-2P170DRAFT_422662
P170DRAFT_82848
CIMG_00407core MAThit onlytblastn27.4cov 34.0%8.0e-0840 bits866,444–866,710 +–5501_rs_MAT_MAT1-2P170DRAFT_422662
SLA2conserved flankhit onlyDIAMOND, proteome87.5cov 100.0%< 1e-1801,568 bits869,849–873,738 -–107463_unknown-1_MAT_MAT1-2P170DRAFT_376660

Withheld candidate loci 1

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (1).

Show the 1 withheld locus

Withheld loci (1)

familylocationidiomorph genesmodelledreason
MATMSFO01000001:4,088,037–4,090,229MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
AsteyniiIBT23096.genome.fasta
Assembly
37 contigs · 37.8 Mb · N50 3.9 Mb
Genome SHA-256
08847ea2f505e9721ccc85c6330d03eee3eb80a6fc606d3f673434c05310a2f3
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:31:43Z
Run time
62 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits