MATPredict · mating-type report

AibericusCBS121593

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:35 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2medium confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on KZ824419MAT1-2 locus: genes from left to right on KZ824419, 1,275,759 to 1,308,601: SLA2 (conserved flank, plus strand); COX13 (conserved flank, minus strand); APN2 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-2-4 (core MAT gene, plus strand); CIMG_00407 (core MAT gene, plus strand).1,280 kb1,290 kb1,300 kb1,310 kb149.7 kb more to contig end →called locusSLA2: conserved flank, 1,275,759–1,279,503 (+), identity 88.5%, search hit onlySLA2COX13: conserved flank, 1,301,862–1,302,565 (-), identity 78.9%, search hit onlyCOX13APN2: conserved flank, 1,302,938–1,305,068 (+), identity 90.9%, search hit onlyAPN2MAT1-1-3: core MAT gene, 1,306,078–1,307,197 (-), identity 35.1%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 1,306,078–1,307,197 (-), identity 55.5%, search hit onlyMAT1-2-1MAT1-2-4: core MAT gene, 1,307,690–1,308,601 (+), identity 47.4%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 1,307,921–1,308,181 (+), identity 30.682%, search hit onlyCIMG_00407
Gene order on KZ824419. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KZ824419, 1,274,117 to 1,310,244BO80DRAFT_420682 1,271,790-1,275,326 (+): Pleckstrin homology domain | Maintenance of mitochondrial morphology protein 1 | domain-containing proteinBO80DRAFT_420682BO80DRAFT_11025 1,275,483-1,279,663 (+): cytoskeleton assembly control protein Sla2BO80DRAFT_11025BO80DRAFT_440257 1,279,862-1,280,612 (+): unspecified productBO80DRAFT_440257BO80DRAFT_440258 1,283,827-1,285,342 (-): FAD/NAD(P)-binding domain-containing proteinBO80DRAFT_440258BO80DRAFT_11157 1,285,333-1,287,451 (-): CoA-transferase family IIIBO80DRAFT_11157BO80DRAFT_420685 1,287,624-1,289,254 (+): putative aldehyde dehydrogenase family proteinBO80DRAFT_420685BO80DRAFT_440261 1,290,173-1,290,457 (+): unspecified productBO80DRAFT_440261BO80DRAFT_420686 1,291,503-1,291,986 (+): unspecified productBO80DRAFT_420686BO80DRAFT_371200 1,292,687-1,294,022 (+): kinase-like proteinBO80DRAFT_371200BO80DRAFT_420688 1,294,807-1,297,003 (+): unspecified productBO80DRAFT_420688BO80DRAFT_460596 1,298,420-1,301,360 (+): anaphase-promoting complex subunit Apc5BO80DRAFT_460596BO80DRAFT_396236 1,301,497-1,302,565 (-): cytochrome c oxidase polypeptide VIaBO80DRAFT_396236BO80DRAFT_489889 1,302,815-1,305,537 (+): DNA lyase Apn2BO80DRAFT_489889BO80DRAFT_396241 1,306,078-1,307,197 (-): mating-type HMG-box protein MAT1-2BO80DRAFT_396241BO80DRAFT_489891 1,307,666-1,308,601 (+): putative mating locus proteinBO80DRAFT_489891 MAT1-2-4BO80DRAFT_11295 1,309,123-1,309,976 (-): unspecified productBO80DRAFT_11295BO80DRAFT_11290 1,309,938-1,310,838 (+): unspecified productBO80DRAFT_11290
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KZ824419:1,275,759–1,308,601 · 32.8 kb
Own genes
KZ824419:1,275,759–1,308,601 · 32.8 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
151.4 kb
Region (samtools)
KZ824419:1275759-1308601
Idiomorph scores
  • MAT1-2 312.0

Margin over the set-aside MAT1-1-3 hit: 20.4 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-155.5MAT1-1-335.1100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome88.5cov 100.0%< 1e-1801,567 bits1,275,759–1,279,503 +–746128_af293_MAT_MAT1-2BO80DRAFT_11025
COX13conserved flankhit onlyDIAMOND, proteome78.9cov 100.0%2.6e-72206 bits1,301,862–1,302,565 -–746128_af293_MAT_MAT1-2BO80DRAFT_396236
APN2conserved flankhit onlyDIAMOND, proteome90.9cov 36.7%< 1e-180917 bits1,302,938–1,305,068 +–2903220_liq80xsp_MAT_combinedBO80DRAFT_489889
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome35.1cov 41.1%4.7e-1257 bits1,306,078–1,307,197 -–230073_uamh-1363_MAT_MAT1-1BO80DRAFT_396241
MAT1-2-1core MAThit onlyDIAMOND, proteome55.5cov 59.2%3.3e-108312 bits1,306,078–1,307,197 -–5501_rs_MAT_MAT1-2BO80DRAFT_396241
MAT1-2-4core MAThit onlyDIAMOND, proteome47.4cov 98.8%5.8e-75221 bits1,307,690–1,308,601 +–746128_af293_MAT_MAT1-2BO80DRAFT_489891 MAT1-2-4
CIMG_00407core MAThit onlytblastn30.7cov 33.0%5.6e-0544 bits1,307,921–1,308,181 +–5501_rs_MAT_MAT1-2BO80DRAFT_489891 MAT1-2-4

Withheld candidate loci 1

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (1).

Show the 1 withheld locus

Withheld loci (1)

familylocationidiomorph genesmodelledreason
MATKZ824425:27,928–30,117MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
AibericusCBS121593.genome.fasta
Assembly
116 contigs · 33.4 Mb · N50 622.4 kb
Genome SHA-256
d91c73a6405b89a29877f02741632b30c845c8be27374b2c1aa60dba83baa7da
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:34:19Z
Run time
59 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits