MATPredict · mating-type report

AglaucusCBS516.65

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:32 UTC by MATPredict 0.8.1

Result

Mating-type loci

MAT1-1medium confidenceMAT1-2medium confidence

Called loci 2

MAT1-1 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-1 locus on KV878907MAT1-1 locus: genes from left to right on KV878907, 5,938 to 10,625: MAT1-2-10 (core MAT gene, minus strand); MAT1-2-4 (core MAT gene, plus strand); CIMG_00407 (core MAT gene, plus strand); MAT1-1-1 (core MAT gene, plus strand).6 kb7 kb8 kb9 kb10 kb← 5.6 kb more to contig endcalled locusMAT1-2-10: core MAT gene, 5,938–6,054 (-), identity 38.462%, search hit onlyMAT1-2-10MAT1-2-4: core MAT gene, 8,270–9,154 (+), identity 48.3%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 8,519–8,764 (+), identity 26.829%, search hit onlyCIMG_00407MAT1-1-1: core MAT gene, 9,683–10,625 (+), identity 52.5%, search hit onlyMAT1-1-1
Gene order on KV878907. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KV878907, 5,638 to 10,925ASPGLDRAFT_763984 5,326-5,959 (+): hypothetical proteinASPGLDRAFT_763984ASPGLDRAFT_763992 7,395-7,923 (-): hypothetical proteinASPGLDRAFT_763992ASPGLDRAFT_133331 8,270-9,154 (+): hypothetical proteinASPGLDRAFT_133331ASPGLDRAFT_89185 9,683-10,625 (+): Mating-type protein MAT alpha 1 HMG-box | domain-containing proteinASPGLDRAFT_89185
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
Location
KV878907:5,938–10,625 · 4.7 kb
Own genes
KV878907:5,938–10,625 · 4.7 kb
Locus
Genes of both idiomorphs at one locus.
Genes modelled
2 of 4 gene hits
Nearest contig end
5.9 kb
Region (samtools)
KV878907:5938-10625
Detection pass
relaxed
Idiomorph scores
  • MAT1-1 249.0
  • MAT1-2 233.0

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-2-10core MAThit onlytblastn38.5cov 19.0%5.5e+0028 bits5,938–6,054 -–40559_sas405_MAT_MAT1-2ASPGLDRAFT_763984 (opposite strand)
MAT1-2-4core MAThit onlyDIAMOND, proteome48.3cov 96.8%1.9e-79233 bits8,270–9,154 +–746128_af293_MAT_MAT1-2ASPGLDRAFT_133331
CIMG_00407core MAThit onlytblastn26.8cov 31.0%3.2e-0235 bits8,519–8,764 +–5501_rs_MAT_MAT1-2ASPGLDRAFT_133331
MAT1-1-1core MAThit onlyDIAMOND, proteome52.5cov 70.6%1.3e-83249 bits9,683–10,625 +–162425_fgsc-a4_MAT_combinedASPGLDRAFT_89185
MAT1-2-1expected, not found–
APN2expected, not found–
SLA2expected, not found–
COX13expected, not found–

MAT1-2 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on KV878943MAT1-2 locus: genes from left to right on KV878943, 104 to 2,126: MAT1-1-3 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-2-4 (core MAT gene, plus strand).0.5 kb1.0 kb1.5 kb2.0 kbcontig endcalled locusMAT1-1-3: core MAT gene, 104–1,295 (-), identity 40.0%, search hit onlyMAT1-1-3MAT1-2-1: core MAT gene, 104–1,295 (-), identity 58.5%, search hit onlyMAT1-2-1MAT1-2-4: core MAT gene, 1,908–2,126 (+), identity 58.3%, search hit onlyMAT1-2-4
Gene order on KV878943. Scroll sideways to see the whole figure.
FungiDB release 71 gene models on KV878943, 1 to 2,426ASPGLDRAFT_40806 104-1,295 (-): HMG (high mobility group) box | domain-containing proteinASPGLDRAFT_40806ASPGLDRAFT_53658 1,646-2,539 (+): hypothetical proteinASPGLDRAFT_53658
FungiDB release 71 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
Location
KV878943:104–2,126 · 2.0 kb
Own genes
KV878943:104–2,126 · 2.0 kb
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 3 gene hits
Nearest contig end
103 bp
Region (samtools)
KV878943:104-2126
Detection pass
relaxed
Idiomorph scores
  • MAT1-2 301.0

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-1-3core MAThit onlyDIAMOND, proteome40.0cov 41.7%2.1e-1464 bits104–1,295 -–230073_uamh-1363_MAT_MAT1-1ASPGLDRAFT_40806
MAT1-2-1core MAThit onlyDIAMOND, proteome58.5cov 65.1%1.7e-103301 bits104–1,295 -–61372_suf959_MAT_MAT1-2ASPGLDRAFT_40806
MAT1-2-4core MAThit onlyDIAMOND, proteome58.3cov 24.0%1.3e-1972 bits1,908–2,126 +–746128_af293_MAT_MAT1-2ASPGLDRAFT_53658
APN2expected, not found–
SLA2expected, not found–
COX13expected, not found–

Withheld candidate loci 26

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (24); too few of the family's genes found (2).

Show the 26 withheld loci

Withheld loci (26)

familylocationidiomorph genesmodelledreason
MATKV878888:533,563–539,891MAT1-2MAT1-2-1, MAT1-2-101fewer than 2 genes could be modelled
MATKV878888:920,616–929,739MAT1-2MAT1-1-2, CIMG_004070fewer than 2 genes could be modelled
MATKV878888:1,380,295–1,403,235MAT1-2MAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATKV878903:113,635–135,904MAT1-2MAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATKV878899:66,006–85,956MAT1-1MAT1-1-5, MAT1-1-40fewer than 2 genes could be modelled
MATKV878899:118,149–131,615MAT1-2MAT1-2-1, MAT1-1-50fewer than 2 genes could be modelled
MATKV878891:55,663–64,372MAT1-2MAT1-2-1, MAT1-2-100fewer than 2 genes could be modelled
MATKV878891:783,935–792,802MAT1-1MAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATKV878895:492,548–493,196undeterminedMAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATKV878895:632,895–643,838MAT1-2MAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATKV878896:158,534–176,345MAT1-2MAT1-2-1, MAT1-1-30fewer than 2 genes could be modelled
MATKV878905:207,525–224,180MAT1-1MAT1-1-1, MAT1-2-40fewer than 2 genes could be modelled
MATKV878905:573,158–592,483MAT1-1MAT1-1-1, MAT1-2-1, MAT1-1-30fewer than 2 genes could be modelled
MATKV878915:24,152–58,625MAT1-2MAT1-1-1, MAT1-2-1, MAT1-2-40fewer than 2 genes could be modelled
MATKV878890:40,602–47,790MAT1-1MAT1-1-2, MAT1-2-70fewer than 2 genes could be modelled
MATKV878890:752,055–764,057MAT1-1MAT1-1-2, MAT1-1-30fewer than 2 genes could be modelled
MATKV878893:29,993–36,636MAT1-1MAT1-1-1, MAT1-1-20fewer than 2 genes could be modelled
MATKV878893:557,717–575,498MAT1-1MAT1-1-1, MAT1-2-100fewer than 2 genes could be modelled
MATKV878910:96,487–126,778MAT1-2MAT1-2-1, MAT1-1-30fewer than 2 genes could be modelled
MATKV878898:148,142–194,614MAT1-1MAT1-1-1, MAT1-2-1, MAT1-1-40fewer than 2 genes could be modelled
MATKV878889:1,054,944–1,065,069MAT1-1MAT1-1-1, MAT1-1-30fewer than 2 genes could be modelled
MATKV878889:1,423,897–1,440,377MAT1-1MAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATKV878912:294,425–359,195MAT1-2MAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATKV878914:294,399–316,599MAT1-1MAT1-1-1, MAT1-1-30fewer than 2 genes could be modelled
MATKV878888:533,563–539,891MAT1-2MAT1-2-1, MAT1-2-101too few of the family's genes found
MATKV878897:287,289–298,003undeterminedSLA2, COX132too few of the family's genes found

Provenance

Genome file
AglaucusCBS516.65.genome.fasta
Assembly
82 contigs · 28.0 Mb · N50 1.0 Mb
Genome SHA-256
05fbb33abb3252d6f97255b86461fcca5c49060d21be7f55d58c5135bbc9fb57
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:31:21Z
Run time
51 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits