MATPredict · mating-type report

RmackenzieiCBS650.93

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:53 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on KN847478MAT1-1 locus: genes from left to right on KN847478, 2,442,225 to 2,452,859: SLA2 (conserved flank, plus strand); MAT1-1-1 (core MAT gene, minus strand); APN2 (conserved flank, minus strand).2,442 kb2,444 kb2,446 kb2,448 kb2,450 kb2,452 kb1,363.8 kb more to contig end →called locusSLA2: conserved flank, 2,442,225–2,445,491 (+), identity 83.6%, search hit onlySLA2MAT1-1-1: core MAT gene, 2,448,348–2,449,242 (-), identity 34.8%, search hit onlyMAT1-1-1APN2: conserved flank, 2,450,980–2,452,859 (-), identity 95.5%, search hit onlyAPN2
Gene order on KN847478. Scroll sideways to see the whole figure.
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
KN847478:2,442,225–2,452,859 · 10.6 kb
Own genes
KN847478:2,442,225–2,452,859 · 10.6 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
3 of 3 gene hits
Nearest contig end
1.4 Mb
Region (samtools)
KN847478:2442225-2452859
Idiomorph scores
  • MAT1-1 88.6

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome83.6cov 100.0%< 1e-1801,459 bits2,442,225–2,445,491 +–1301501_voucher-215_MAT_MAT1-1Z518_06023
MAT1-1-1core MAThit onlyDIAMOND, proteome34.8cov 36.3%1.4e-2289 bits2,448,348–2,449,242 -–162425_fgsc-a4_MAT_combinedZ518_06024
APN2conserved flankhit onlyDIAMOND, proteome95.5cov 36.7%9.4e-244680 bits2,450,980–2,452,859 -–2903220_liq80xsp_MAT_combinedZ518_06025
COX13expected, not found–

Withheld candidate loci 4

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (4).

Show the 4 withheld loci
familylocationidiomorph genesmodelledreason
MATKN847477:1,835,135–1,836,259undeterminedAPN21too few of the family's genes found
MATKN847478:1,103,381–1,127,208MAT1-1MAT1-1-1, MAT1-2-1, MAT1-1-21too few of the family's genes found
MATKN847478:2,564,975–2,565,593undeterminedCOX131too few of the family's genes found
MATKN847480:707,210–710,100MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
RmackenzieiCBS650.93.genome.fasta
Assembly
17 contigs · 32.5 Mb · N50 3.8 Mb
Genome SHA-256
6cde4074869b609c572b1a333ba1bf2186a64d44da7df796a836cb505d744a6c
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 43219, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 43219', 'routed_by': 'family taxid 43219 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:52:42Z
Run time
54 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits