MATPredict · mating-type report

Pstriiformis93-210

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:02 UTC by MATPredict 0.8.1

Result

Mating-type loci

undeterminedhigh confidenceundeterminedhigh confidence

Called loci 2

undetermined rustHD family · Basidiomycota

high confidence
Gene order of the undetermined locus on PKSL01000053undetermined locus: genes from left to right on PKSL01000053, 77,397 to 81,261: bW (core MAT gene, minus strand); bE (core MAT gene, plus strand).78 kb79 kb80 kb81 kb← 77.1 kb more to contig endcalled locusbW: core MAT gene, 77,397–79,584 (-), identity 66.4%, search hit onlybWbE: core MAT gene, 79,845–81,261 (+), identity 72.5%, search hit onlybE
Gene order on PKSL01000053. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on PKSL01000053, 77,097 to 81,561PSTT_06650 77,397-79,584 (-): Homeobox KN domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A2S4VJA6]PSTT_06650PSTT_06651 79,845-81,261 (+): Homeobox domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A2S4VJB7]PSTT_06651
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
PKSL01000053:77,397–81,261 · 3.9 kb
Own genes
PKSL01000053:77,397–81,261 · 3.9 kb
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
77.4 kb
Region (samtools)
PKSL01000053:77397-81261

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
bWcore MAThit onlyDIAMOND, proteome66.4cov 100.0%1.2e-232647 bits77,397–79,584 -–1165861_pst-78_rustHD_b1PSTT_06650
bEcore MAThit onlyDIAMOND, proteome72.5cov 99.3%9.1e-174484 bits79,845–81,261 +–1165861_pst-78_rustHD_b1PSTT_06651

undetermined rustHD family · Basidiomycota

high confidence
Gene order of the undetermined locus on PKSL01000113undetermined locus: genes from left to right on PKSL01000113, 20,528 to 24,466: bE (core MAT gene, minus strand); bW (core MAT gene, plus strand).21 kb22 kb23 kb24 kb← 20.2 kb more to contig endcalled locusbE: core MAT gene, 20,528–21,971 (-), identity 75.6%, search hit onlybEbW: core MAT gene, 22,589–24,466 (+), identity 85.7%, search hit onlybW
Gene order on PKSL01000113. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on PKSL01000113, 20,228 to 24,766PSTT_10472 20,528-21,971 (-): Homeobox domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A2S4V454]PSTT_10472PSTT_10473 22,589-24,466 (+): Homeobox KN domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A2S4V457]PSTT_10473
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
  • Idiomorph undetermined: the evidence for two idiomorphs is tied or too close to call.
Location
PKSL01000113:20,528–24,466 · 3.9 kb
Own genes
PKSL01000113:20,528–24,466 · 3.9 kb
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
20.5 kb
Region (samtools)
PKSL01000113:20528-24466

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
bEcore MAThit onlyDIAMOND, proteome75.6cov 100.0%8.1e-182505 bits20,528–21,971 -–1165861_pst-78_rustHD_b1PSTT_10472
bWcore MAThit onlyDIAMOND, proteome85.7cov 98.8%1.5e-285781 bits22,589–24,466 +–1165861_pst-78_rustHD_b1PSTT_10473

Withheld candidate loci 13

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (13).

Show the 13 withheld loci

Withheld loci (13)

familylocationidiomorph genesmodelledreason
rustHDPKSL01000053:133,222–134,603undeterminedbE1fewer than 2 genes could be modelled
rustHDPKSL01000024:420,742–420,867undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000024:452,411–452,686undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000041:450,119–450,328undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000081:24,510–25,031undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000172:49,406–49,513undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000083:223,362–223,604undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000093:18,571–18,810undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000085:75,168–75,266undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000084:207,802–207,936undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000197:77,716–78,105undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000011:461,685–461,837undeterminedbW0fewer than 2 genes could be modelled
rustHDPKSL01000021:95,680–95,820undeterminedbW0fewer than 2 genes could be modelled

Provenance

Genome file
Pstriiformis93-210.genome.fasta
Assembly
493 contigs · 84.6 Mb · N50 295.4 kb
Genome SHA-256
32c2dad71f1aeac8d03fed39a43a6d72b9ad1a544e175f7c77104e4a6a4ebb11
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'genus', 'routing_taxid': 5262, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 5262', 'routed_by': 'family taxid 5262 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T02:01:55Z
Run time
35 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits