MATPredict · mating-type report

PomphalodesCBS100304

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:52 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-2 MATtub family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on JAJJXM010000056MAT1-2 locus: genes from left to right on JAJJXM010000056, 335,693 to 348,245: MAT1-2-1 (core MAT gene, minus strand); APN2 (conserved flank, minus strand).336 kb338 kb340 kb342 kb344 kb346 kb348 kb103.2 kb more to contig end →called locusMAT1-2-1: core MAT gene, 335,693–336,672 (-), identity 45.3%, search hit onlyMAT1-2-1APN2: conserved flank, 346,192–348,245 (-), identity 68.8%, search hit onlyAPN2
Gene order on JAJJXM010000056. Scroll sideways to see the whole figure.
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
JAJJXM010000056:335,693–348,245 · 12.6 kb
Own genes
JAJJXM010000056:335,693–348,245 · 12.6 kb
Locus
Provisional: the call only tied the admission bar, or came from the relaxed pass.
Genes modelled
2 of 2 gene hits
Nearest contig end
103.9 kb
Region (samtools)
JAJJXM010000056:335693-348245
Detection pass
relaxed
Idiomorph scores
  • MAT1-2 82.4

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-2-1core MAThit onlyDIAMOND, proteome45.3cov 53.4%1.3e-2182 bits335,693–336,672 -–55307_ysh1-8_MATtub_MAT1-2BZA77DRAFT_323747
APN2conserved flankhit onlyDIAMOND, proteome68.8cov 89.2%1.3e-126363 bits346,192–348,245 -–1174673_ypl6-1_MATtub_MAT1-2BZA77DRAFT_286141
Rsm22-likeexpected, not found–
unnamed_flanking_ORFexpected, not found–

Withheld candidate loci 4

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3); fewer than 2 genes could be modelled (1).

Show the 4 withheld loci
familylocationidiomorph genesmodelledreason
MATtubJAJJXM010000006:79,652–119,626MAT1-2MAT1-1-1, MAT1-2-10fewer than 2 genes could be modelled
MATtubJAJJXM010000012:461,680–463,765MAT1-2MAT1-2-11too few of the family's genes found
MATtubJAJJXM010000033:155,909–160,018undeterminedSLA21too few of the family's genes found
MATtubJAJJXM010000064:240,867–243,335undeterminedRsm22-like1too few of the family's genes found

Provenance

Genome file
PomphalodesCBS100304.genome.fasta
Assembly
260 contigs · 61.0 Mb · N50 709.5 kb
Genome SHA-256
33ebdb49d96e50fca85f5f05e7e00298c8cd26edbf407ad41804927c5e354a24
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 110846, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 110846', 'routed_by': 'family taxid 110846 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T01:51:53Z
Run time
46 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits