MATPredict · mating-type report

PmurinaB123

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:50 UTC by MATPredict 0.8.1

Result

Mating type

Pmedium confidence

Called loci 1

P PM family · Ascomycota

medium confidence
Gene order of the P locus on AFWA02000013P locus: genes from left to right on AFWA02000013, 71,308 to 80,799: matPi (core MAT gene, minus strand); matMc (core MAT gene, minus strand).72 kb74 kb76 kb78 kb80 kb← 70.8 kb more to contig endcalled locusmatPi: core MAT gene, 71,308–72,000 (-), identity 90.0%, search hit onlymatPimatMc: core MAT gene, 80,110–80,799 (-), identity 85.8%, search hit onlymatMc
Gene order on AFWA02000013. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on AFWA02000013, 70,834 to 81,273PNEG_02273 71,308-72,000 (-): Homeobox domain-containing protein [Source:UniProtKB/TrEMBL;Acc:M7P643]PNEG_02273PNEG_02274 72,135-75,851 (+): ATP-dependent chaperone ClpBPNEG_02274PNEG_04001 75,852-79,754 (+): I/LWEQ domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A0W4ZWW1]PNEG_04001PNEG_02275 80,110-80,799 (-): HMG box domain-containing protein [Source:UniProtKB/TrEMBL;Acc:M7NPY5]PNEG_02275
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
Location
AFWA02000013:71,308–80,799 · 9.5 kb
Cross-lineage class
unassigned
Own genes
AFWA02000013:71,308–80,799 · 9.5 kb
Locus
Genes of both idiomorphs at one locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
71.3 kb
Region (samtools)
AFWA02000013:71308-80799
Idiomorph scores
  • P 410.0
  • M 385.0

Margin over the next idiomorph: 25.0

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
matPicore MAThit onlyDIAMOND, proteome90.0cov 100.0%1.8e-151410 bits71,308–72,000 -–4754_b80_PM_combinedPNEG_02273
matMccore MAThit onlyDIAMOND, proteome85.8cov 100.0%7.4e-142385 bits80,110–80,799 -–4754_b80_PM_combinedPNEG_02275
matMiexpected, not found–
matPcnot searchable: no reference protein in the database–

Provenance

Genome file
PmurinaB123.genome.fasta
Assembly
20 contigs · 7.5 Mb · N50 492.8 kb
Genome SHA-256
609328a97681ee9a0738c874a32e39a9474f3a08d564394d09d255114f8e02d5
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 44281, 'routing_taxid_rank': 'family', 'flags': ['short_genome', 'identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 44281', 'routed_by': 'family taxid 44281 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T01:50:31Z
Run time
12 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits