MATPredict · mating-type report

McanisCBS113480

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:48 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1medium confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-1 locus on DS995708MAT1-1 locus: genes from left to right on DS995708, 399,744 to 411,950: CIMG_00407 (core MAT gene, minus strand); MAT1-2-4 (core MAT gene, minus strand); MAT1-1-1 (core MAT gene, minus strand); MAT1-1-4 (core MAT gene, minus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand); SLA2 (conserved flank, minus strand).400 kb402 kb404 kb406 kb408 kb410 kb412 kb← 399.1 kb more to contig endcalled locusCIMG_00407: core MAT gene, 399,744–400,382 (-), identity 26.761%, search hit onlyCIMG_00407MAT1-2-4: core MAT gene, 399,858–400,019 (-), identity 40.74%, gene modelMAT1-2-4MAT1-1-1: core MAT gene, 400,694–401,886 (-), identity 47.6%, search hit onlyMAT1-1-1MAT1-1-4: core MAT gene, 402,765–403,997 (-), identity 48.0%, search hit onlyMAT1-1-4APN2: conserved flank, 404,654–406,484 (-), identity 76.6%, search hit onlyAPN2COX13: conserved flank, 406,957–407,612 (+), identity 64.6%, search hit onlyCOX13SLA2: conserved flank, 408,009–411,950 (-), identity 88.1%, search hit onlySLA2
Gene order on DS995708. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on DS995708, 399,134 to 412,560MCYG_08068 399,639-400,583 (-): unspecified productMCYG_08068MCYG_08069 400,694-401,886 (-): alpha-box mating type proteinMCYG_08069MCYG_08070 402,765-403,997 (-): conserved hypothetical proteinMCYG_08070MCYG_08071 404,654-406,484 (-): ap endonucleaseMCYG_08071MCYG_08072 406,957-407,612 (+): Cox13pMCYG_08072MCYG_08073 408,009-411,962 (-): SlaBMCYG_08073MCYG_08074 412,500-415,241 (-): uncharacterized PH domain-containing proteinMCYG_08074
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • gene model
  • search hit only
Location
DS995708:399,744–411,950 · 12.2 kb
Own genes
DS995708:399,744–411,950 · 12.2 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
6 of 7 gene hits
Nearest contig end
399.7 kb
Region (samtools)
DS995708:399744-411950
Idiomorph scores
  • MAT1-1 295.0
  • MAT1-2 70.1

Margin over the next idiomorph: 224.9

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
CIMG_00407core MAThit onlytblastn26.8cov 61.0%1.3e-1370 bits399,744–400,382 -–5501_rs_MAT_MAT1-2MCYG_08068
MAT1-2-4core MATgene modelexonerate40.7cov 21.6%2.4e-1060 bits399,858–400,019 -1746128_af293_MAT_MAT1-2MCYG_08068
MAT1-1-1core MAThit onlyDIAMOND, proteome47.6cov 99.2%5.3e-100295 bits400,694–401,886 -–199306_rmscc1040_MAT_MAT1-1MCYG_08069
MAT1-1-4core MAThit onlyDIAMOND, proteome48.0cov 38.6%9.3e-2489 bits402,765–403,997 -–199306_rmscc1040_MAT_MAT1-1MCYG_08070
APN2conserved flankhit onlyDIAMOND, proteome76.6cov 90.4%2.0e-223627 bits404,654–406,484 -–27339_unknown-1_MAT_MAT1-2MCYG_08071
COX13conserved flankhit onlyDIAMOND, proteome64.6cov 100.0%1.8e-53158 bits406,957–407,612 +–5501_rs_MAT_MAT1-2MCYG_08072
SLA2conserved flankhit onlyDIAMOND, proteome88.1cov 99.3%< 1e-1801,576 bits408,009–411,950 -–107463_unknown-1_MAT_MAT1-2MCYG_08073
MAT1-2-1expected, not found–

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATDS995702:396,710–398,536MAT1-1MAT1-1-21too few of the family's genes found
MATDS995706:775,592–822,061MAT1-2MAT1-1-1, MAT1-2-1, MAT1-2-101too few of the family's genes found

Provenance

Genome file
McanisCBS113480.genome.fasta
Assembly
17 contigs · 23.3 Mb · N50 2.9 Mb
Genome SHA-256
502e3737fe3bcf82c72989841fa4bc2032b136155505795c55e710b76294bd02
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 34384, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 34384', 'routed_by': 'family taxid 34384 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:47:10Z
Run time
66 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits