MATPredict · mating-type report

ManisopliaeARSEF549

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:12 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1medium confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-1 locus on AZNF01000002MAT1-1 locus: genes from left to right on AZNF01000002, 2,798,468 to 2,828,979: MAT1-2-1 (core MAT gene, plus strand); SLA2 (conserved flank, plus strand); MAT1-1-1 (core MAT gene, minus strand); MAT1-1-2 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).2,800 kb2,805 kb2,810 kb2,815 kb2,820 kb2,825 kb2,830 kb1,371.0 kb more to contig end →called locusMAT1-2-1: core MAT gene, 2,798,468–2,798,566 (+), identity 39.394%, search hit onlyMAT1-2-1 (lost to MAT1-1-3)SLA2: conserved flank, 2,807,218–2,811,564 (+), identity 80.3%, search hit onlySLA2MAT1-1-1: core MAT gene, 2,819,478–2,820,608 (-), identity 44.5%, search hit onlyMAT1-1-1MAT1-1-2: core MAT gene, 2,822,131–2,823,180 (-), identity 28.5%, search hit onlyMAT1-1-2MAT1-1-3: core MAT gene, 2,823,484–2,824,177 (+), identity 46.4%, search hit onlyMAT1-1-3APN2: conserved flank, 2,825,541–2,827,591 (-), identity 75.0%, search hit onlyAPN2COX13: conserved flank, 2,828,248–2,828,979 (+), identity 66.2%, search hit onlyCOX13
Gene order on AZNF01000002. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on AZNF01000002, 2,796,945 to 2,830,505MAN_02383 2,796,152-2,801,303 (-): DNA polymerase family BMAN_02383MAN_02384 2,802,004-2,803,383 (+): pre-mRNA-splicing factor slu7MAN_02384MAN_02385 2,803,718-2,804,434 (-): RNA-binding S4MAN_02385MAN_02386 2,804,834-2,805,564 (+): unspecified productMAN_02386MAN_02387 2,807,218-2,811,564 (+): cytoskeleton assembly control protein Sla2MAN_02387MAN_02388 2,819,478-2,820,608 (-): mating-type A-1 proteinMAN_02388MAN_10891 2,822,131-2,823,180 (-): mat1-1-2MAN_10891MAN_02389 2,823,484-2,824,177 (+): MAT1-1-3 like proteinMAN_02389MAN_02390 2,825,541-2,827,591 (-): DNA lyaseMAN_02390MAN_02391 2,828,248-2,828,979 (+): Cytochrome c oxidase, subunit VIaMAN_02391MAN_02392 2,829,706-2,832,180 (+): anaphase-promoting complex proteinMAN_02392
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
AZNF01000002:2,798,468–2,828,979 · 30.5 kb
Own genes
AZNF01000002:2,798,468–2,828,979 · 30.5 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
6 of 7 gene hits
Nearest contig end
1.4 Mb
Region (samtools)
AZNF01000002:2798468-2828979
Idiomorph scores
  • MAT1-1 184.0
  • MAT1-2 28.9

Margin over the next idiomorph: 155.1

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-1-346.4MAT1-2-129.2100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-2-1core MATlost to MAT1-1-3, not countedhit onlytblastn39.4cov 10.0%6.2e+0029 bits2,798,468–2,798,566 +–162425_fgsc-a4_MAT_combinedMAN_02383 (opposite strand)
SLA2conserved flankhit onlyDIAMOND, proteome80.3cov 100.0%< 1e-1801,361 bits2,807,218–2,811,564 +–107463_unknown-1_MAT_MAT1-2MAN_02387
MAT1-1-1core MAThit onlyDIAMOND, proteome44.5cov 31.3%6.7e-58184 bits2,819,478–2,820,608 -–2903222_liq146xsp_MAT_combinedMAN_02388
MAT1-1-2core MAThit onlyDIAMOND, proteome28.5cov 83.1%1.7e-35125 bits2,822,131–2,823,180 -–5127_mo44_MAT_MAT1-1MAN_10891
MAT1-1-3core MAThit onlyDIAMOND, proteome46.4cov 96.7%4.2e-58173 bits2,823,484–2,824,177 +–5518_3639_MAT_combinedMAN_02389
APN2conserved flankhit onlyDIAMOND, proteome75.0cov 13.3%6.6e-195555 bits2,825,541–2,827,591 -–2903220_liq80xsp_MAT_combinedMAN_02390
COX13conserved flankhit onlyDIAMOND, proteome66.2cov 100.0%8.2e-62179 bits2,828,248–2,828,979 +–578113_sxlc146_MAT_MAT1-2MAN_02391

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATAZNF01000010:762,903–765,154MAT1-2MAT1-2-11too few of the family's genes found
MATAZNF01000011:850,972–874,365MAT1-1MAT1-1-1, MAT1-1-31too few of the family's genes found

Provenance

Genome file
ManisopliaeARSEF549.genome.fasta
Assembly
74 contigs · 38.5 Mb · N50 2.0 Mb
Genome SHA-256
2a816b642b6b0964275fb120ce64ba5e7083c70f33e5dfcb359a32580cc81b70
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 34397, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 34397', 'routed_by': 'family taxid 34397 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:11:29Z
Run time
80 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits