MATPredict · mating-type report

EdermatitidisNIHUT8656

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:04 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2high confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on JH226137MAT1-2 locus: genes from left to right on JH226137, 289,121 to 308,133: SLA2 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand).290 kb295 kb300 kb305 kb← 288.2 kb more to contig endcalled locusSLA2: conserved flank, 289,121–292,377 (+), identity 83.6%, search hit onlySLA2MAT1-1-3: core MAT gene, 303,111–304,250 (+), identity 32.4%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 303,111–304,250 (+), identity 68.9%, search hit onlyMAT1-2-1APN2: conserved flank, 306,174–308,133 (-), identity 93.2%, search hit onlyAPN2
Gene order on JH226137. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on JH226137, 288,170 to 309,083HMPREF1120_08857 283,752-288,695 (-): unspecified productHMPREF1120_08857HMPREF1120_08859 289,121-292,377 (+): huntingtin interacting protein 1HMPREF1120_08859HMPREF1120_08860 293,343-295,924 (-): unspecified productHMPREF1120_08860HMPREF1120_09286 295,802-295,917 (+): 5S ribosomal RNAHMPREF1120_09286HMPREF1120_08861 299,296-300,103 (-): unspecified productHMPREF1120_08861HMPREF1120_08862 303,111-304,250 (+): unspecified productHMPREF1120_08862HMPREF1120_08863 305,794-308,458 (-): AP endonuclease 2HMPREF1120_08863HMPREF1120_08864 308,976-318,584 (+): unspecified productHMPREF1120_08864
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
JH226137:289,121–308,133 · 19.0 kb
Own genes
JH226137:289,121–308,133 · 19.0 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
3 of 4 gene hits
Nearest contig end
289.1 kb
Region (samtools)
JH226137:289121-308133
Idiomorph scores
  • MAT1-2 125.0

Margin over the set-aside MAT1-1-3 hit: 36.5 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-168.9MAT1-1-332.4100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome83.6cov 100.0%< 1e-1801,441 bits289,121–292,377 +–107463_unknown-1_MAT_MAT1-2HMPREF1120_08859
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome32.4cov 41.1%2.5e-0949 bits303,111–304,250 +–230073_uamh-1363_MAT_MAT1-1HMPREF1120_08862
MAT1-2-1core MAThit onlyDIAMOND, proteome68.9cov 26.6%3.4e-35125 bits303,111–304,250 +–199306_silveira_MAT_MAT1-2HMPREF1120_08862
APN2conserved flankhit onlyDIAMOND, proteome93.2cov 36.7%2.4e-243680 bits306,174–308,133 -–2903220_liq80xsp_MAT_combinedHMPREF1120_08863
COX13expected, not found–

Withheld candidate loci 3

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3).

Show the 3 withheld loci

Withheld loci (3)

familylocationidiomorph genesmodelledreason
MATJH226132:2,143,810–2,162,231MAT1-2MAT1-2-1, MAT1-1-21too few of the family's genes found
MATJH226134:347,028–350,442MAT1-1MAT1-1-31too few of the family's genes found
MATJH226137:138,997–139,593undeterminedCOX131too few of the family's genes found

Provenance

Genome file
EdermatitidisNIHUT8656.genome.fasta
Assembly
11 contigs · 26.4 Mb · N50 3.6 Mb
Genome SHA-256
7cad5aafac49a8b2ba53138a5811c7f20aba00dd973cd93aa6f0134d9b94869f
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 43219, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 43219', 'routed_by': 'family taxid 43219 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:03:29Z
Run time
61 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits