MATPredict · mating-type report

CgattiiEJB2

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:56 UTC by MATPredict 0.8.1

Result

Mating type

alphamedium confidence

Called loci 1

alpha MAT family · Basidiomycota

medium confidence
Gene order of the alpha locus on KN848799alpha locus: genes from left to right on KN848799, 4,771 to 11,162: STE3 (core MAT gene, plus strand); MFalpha (core MAT gene, minus strand).5 kb6 kb7 kb8 kb9 kb10 kb11 kbcontig endcalled locusSTE3: core MAT gene, 4,771–6,089 (+), identity 90.8%, search hit onlySTE3MFalpha: core MAT gene, 11,070–11,162 (-), identity 93.3%, search hit onlyMFalpha
Gene order on KN848799. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on KN848799, 4,452 to 11,164I306_06608 4,771-6,089 (+): pheromone a factor receptorI306_06608I306_06609 7,275-9,938 (+): IKS protein kinaseI306_06609I306_06610 11,070-11,162 (-): hypothetical proteinI306_06610
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • search hit only
Location
KN848799:4,771–11,162 · 6.4 kb
Own genes
KN848799:4,771–11,162 · 6.4 kb
Locus
A MAT gene with no flanking gene beside it; not a full locus.
Genes modelled
2 of 2 gene hits
Nearest contig end
2 bp
Region (samtools)
KN848799:4771-11162
Idiomorph scores
  • alpha 57.4

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
STE3core MAThit onlyDIAMOND, proteome90.8cov 100.0%1.2e-262704 bits4,771–6,089 +–40410_jec21_MAT_alphaI306_06608
MFalphacore MAThit onlyDIAMOND, proteome93.3cov 78.9%1.4e-1757 bits11,070–11,162 -–40410_jec21_MAT_alphaI306_06610
FAO1expected, not found–
PAN6expected, not found–
NOG2not searchable: no reference protein in the database–
UAP1not searchable: no reference protein in the database–

Withheld candidate loci 6

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (6).

Show the 6 withheld loci

Withheld loci (6)

familylocationidiomorph genesmodelledreason
MATKN848661:260,248–261,676undeterminedSTE31too few of the family's genes found
MATKN848795:34,496–38,352alphaFAO1, SXI1, SXI23too few of the family's genes found
MATKN848800:254–370alphaMFalpha1too few of the family's genes found
MATKN848801:1,256–1,372alphaMFalpha1too few of the family's genes found
MATKN848803:5,444–5,943undeterminedRPL391too few of the family's genes found
MATKN848804:3,519–5,200undeterminedPAN61too few of the family's genes found

Provenance

Genome file
CgattiiEJB2.genome.fasta
Assembly
282 contigs · 17.7 Mb · N50 225.1 kb
Genome SHA-256
862041ac1f1ef5f4bee150a0a8fa520e930df71f6f7f14694b1d3a5175c2578e
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Basidiomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Basidiomycota', 'phylum_taxid': 5204, 'deepest_rank': 'genus', 'routing_taxid': 1884633, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Basidiomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1884633', 'routed_by': 'family taxid 1884633 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, supported_min_bitscore=33.0
Run started
2026-11-07T01:55:59Z
Run time
23 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits