MATPredict · mating-type report

BbassianaHN6

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:54 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2high confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on CP045886MAT1-2 locus: genes from left to right on CP045886, 208,122 to 225,374: SLA2 (conserved flank, plus strand); MAT1-2-1 (core MAT gene, plus strand); MAT1-1-3 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).210 kb215 kb220 kb225 kb← 207.3 kb more to contig endcalled locusSLA2: conserved flank, 208,122–211,584 (+), identity 83.06%, gene modelSLA2MAT1-2-1: core MAT gene, 219,803–220,334 (+), identity 37.74%, the two modelling tools disagreeMAT1-2-1MAT1-1-3: core MAT gene, 219,998–220,090 (+), identity 41.935%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)APN2: conserved flank, 221,986–224,200 (-), identity 52.48%, the two modelling tools disagreeAPN2COX13: conserved flank, 224,622–225,374 (+), identity 67.18%, the two modelling tools disagreeCOX13
Gene order on CP045886. Scroll sideways to see the whole figure.
  • core MAT gene
  • conserved flank
  • gene model
  • tools disagree
  • alternate model
  • search hit only
Location
CP045886:208,122–225,374 · 17.3 kb
Own genes
CP045886:208,122–225,374 · 17.3 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
4 of 5 gene hits
Nearest contig end
208.1 kb
Region (samtools)
CP045886:208122-225374
Idiomorph scores
  • MAT1-2 76.3

Margin over the set-aside MAT1-1-3 hit: 16.9 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-158.8MAT1-1-341.990.3higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference record
SLA2conserved flankgene modelexonerate83.1cov 99.7%< 1e-1801,618 bits208,122–211,584 +4578113_sxlc146_MAT_MAT1-2
MAT1-2-1core MATmodels differexonerateminiprot: 219,680–220,358, 40.2%37.7cov 69.4%4.0e-2176 bits219,803–220,334 +227337_vdls-17_MAT_MAT1-2
MAT1-1-3core MATlost to MAT1-2-1, not countedhit onlytblastn41.9cov 17.0%2.0e-0332 bits219,998–220,090 +–230073_uamh-1363_MAT_MAT1-1
APN2conserved flankmodels differexonerateminiprot: 221,980–224,200, 51.5%52.5cov 99.8%6.0e-168533 bits221,986–224,200 -6162425_fgsc-a4_MAT_combined
COX13conserved flankmodels differexonerateminiprot: 224,622–225,383, 68.4%67.2cov 100.0%4.2e-2599 bits224,622–225,374 +427337_vdls-17_MAT_MAT1-2

Withheld candidate loci 16

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: fewer than 2 genes could be modelled (16).

Show the 16 withheld loci

Withheld loci (16)

familylocationidiomorph genesmodelledreason
MATCP045886:2,061,788–2,083,604MAT1-2MAT1-2-1, APN20fewer than 2 genes could be modelled
MATCP045886:2,125,443–2,150,124MAT1-1MAT1-1-1, APN20fewer than 2 genes could be modelled
MATCP045886:6,150,500–6,170,778MAT1-2MAT1-1-1, MAT1-2-1, APN20fewer than 2 genes could be modelled
MATCP045880:988,883–992,529MAT1-2MAT1-2-1, APN20fewer than 2 genes could be modelled
MATCP045881:68,126–91,615MAT1-1MAT1-1-1, APN20fewer than 2 genes could be modelled
MATCP045881:1,722,783–1,733,974MAT1-2MAT1-1-1, MAT1-2-1, COX130fewer than 2 genes could be modelled
MATCP045881:2,754,186–2,769,593MAT1-1MAT1-1-1, SLA20fewer than 2 genes could be modelled
MATCP045881:3,019,794–3,038,990MAT1-1MAT1-1-1, APN20fewer than 2 genes could be modelled
MATCP045881:3,251,294–3,260,590MAT1-1MAT1-1-1, COX130fewer than 2 genes could be modelled
MATCP045881:3,428,452–3,438,429MAT1-2MAT1-2-1, SLA20fewer than 2 genes could be modelled
MATCP045884:1,655,602–1,672,140MAT1-1MAT1-1-1, APN20fewer than 2 genes could be modelled
MATCP045884:2,445,310–2,464,859MAT1-1MAT1-1-1, APN20fewer than 2 genes could be modelled
MATCP045883:3,471,051–3,495,653MAT1-2MAT1-2-1, COX130fewer than 2 genes could be modelled
MATCP045882:564,832–571,946MAT1-1MAT1-1-1, APN20fewer than 2 genes could be modelled
MATCP045879:1,324,364–1,348,371MAT1-2MAT1-2-1, APN20fewer than 2 genes could be modelled
MATCP045878:75,231–95,248MAT1-1MAT1-1-1, COX130fewer than 2 genes could be modelled

Provenance

Genome file
BbassianaHN6.genome.fasta
Assembly
12 contigs · 37.1 Mb · N50 4.6 Mb
Genome SHA-256
d1739c203929bf202110e2cb99dda53c5710e99adb7c730819096c9380bd413e
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 474943, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 474943', 'routed_by': 'family taxid 474943 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:52:53Z
Run time
98 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits