MATPredict · mating-type report

AsclerotiicarbonariusCBS121057

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:11 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2medium confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on KZ826400MAT1-2 locus: genes from left to right on KZ826400, 62,548 to 94,835: SLA2 (conserved flank, plus strand); COX13 (conserved flank, minus strand); APN2 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, minus strand); MAT1-2-4 (core MAT gene, plus strand); CIMG_00407 (core MAT gene, plus strand).70 kb80 kb90 kb53.0 kb more to contig end →called locusSLA2: conserved flank, 62,548–66,308 (+), identity 90.2%, search hit onlySLA2COX13: conserved flank, 88,138–88,845 (-), identity 76.1%, search hit onlyCOX13APN2: conserved flank, 89,475–91,368 (+), identity 88.6%, search hit onlyAPN2MAT1-1-3: core MAT gene, 92,373–93,486 (-), identity 31.3%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 92,373–93,486 (-), identity 54.2%, search hit onlyMAT1-2-1MAT1-2-4: core MAT gene, 93,975–94,835 (+), identity 46.7%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 94,206–94,460 (+), identity 30.233%, search hit onlyCIMG_00407
Gene order on KZ826400. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on KZ826400, 60,931 to 96,452BO78DRAFT_400753 58,651-62,069 (+): SMP-LTD domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A319F8R9]BO78DRAFT_400753BO78DRAFT_377597 62,330-66,472 (+): cytoskeleton assembly control protein Sla2BO78DRAFT_377597BO78DRAFT_390508 66,704-67,368 (+): F-box domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A319E1J3]BO78DRAFT_390508BO78DRAFT_325919 70,718-72,248 (-): FAD/NAD(P)-binding domain-containing proteinBO78DRAFT_325919BO78DRAFT_228072 72,329-74,380 (-): CoA-transferase family IIIBO78DRAFT_228072BO78DRAFT_400759 74,546-76,133 (+): putative aldehyde dehydrogenase family proteinBO78DRAFT_400759BO78DRAFT_454524 77,017-78,190 (-): YVTN repeat-like/Quino protein amine dehydrogenaseBO78DRAFT_454524BO78DRAFT_454527 79,515-80,415 (-): NAD(P)-binding proteinBO78DRAFT_454527BO78DRAFT_325838 82,695-83,159 (-): heterokaryon incompatibilityBO78DRAFT_325838BO78DRAFT_352208 84,609-87,635 (+): anaphase-promoting complex subunit Apc5BO78DRAFT_352208BO78DRAFT_352213 87,698-88,845 (-): mitochondrial cytochrome c oxidase subunit VIaBO78DRAFT_352213BO78DRAFT_400761 89,140-91,449 (+): DNA lyase Apn2BO78DRAFT_400761BO78DRAFT_454533 92,373-93,559 (-): mating-type HMG-box protein MAT1-2BO78DRAFT_454533BO78DRAFT_325892 93,975-94,835 (+): putative mating locus proteinBO78DRAFT_325892BO78DRAFT_228165 95,320-96,176 (-): unspecified productBO78DRAFT_228165
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KZ826400:62,548–94,835 · 32.3 kb
Own genes
KZ826400:62,548–94,835 · 32.3 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
54.6 kb
Region (samtools)
KZ826400:62548-94835
Idiomorph scores
  • MAT1-2 298.0

Margin over the set-aside MAT1-1-3 hit: 22.9 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-154.2MAT1-1-331.3100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome90.2cov 100.0%< 1e-1801,617 bits62,548–66,308 +–746128_af293_MAT_MAT1-2BO78DRAFT_377597
COX13conserved flankhit onlyDIAMOND, proteome76.1cov 100.0%2.0e-69199 bits88,138–88,845 -–746128_af293_MAT_MAT1-2BO78DRAFT_352213
APN2conserved flankhit onlyDIAMOND, proteome88.6cov 36.7%4.2e-309845 bits89,475–91,368 +–2903220_liq80xsp_MAT_combinedBO78DRAFT_400761
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome31.3cov 41.1%1.4e-1052 bits92,373–93,486 -–230073_uamh-1363_MAT_MAT1-1BO78DRAFT_454533
MAT1-2-1core MAThit onlyDIAMOND, proteome54.2cov 25.6%1.8e-102298 bits92,373–93,486 -–115814_voucher-101_MAT_MAT1-2BO78DRAFT_454533
MAT1-2-4core MAThit onlyDIAMOND, proteome46.7cov 96.8%1.8e-73218 bits93,975–94,835 +–746128_af293_MAT_MAT1-2BO78DRAFT_325892
CIMG_00407core MAThit onlytblastn30.2cov 32.0%4.8e-0442 bits94,206–94,460 +–5501_rs_MAT_MAT1-2BO78DRAFT_325892

Provenance

Genome file
AsclerotiicarbonariusCBS121057.genome.fasta
Assembly
166 contigs · 37.6 Mb · N50 362.8 kb
Genome SHA-256
95be2840abb89cc8dccf96411016303b4d2dc1d27c578fdade5d9e3333acb2af
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:10:36Z
Run time
71 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits