MATPredict · mating-type report

AnovofumigatusIBT16806

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:06 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on MSZS01000006MAT1-2 locus: genes from left to right on MSZS01000006, 938,973 to 952,103: SLA2 (conserved flank, plus strand); MAT1-2-4 (core MAT gene, minus strand); CIMG_00407 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).940 kb945 kb950 kb← 938.3 kb more to contig endcalled locusSLA2: conserved flank, 938,973–942,872 (+), identity 97.7%, search hit onlySLA2MAT1-2-4: core MAT gene, 945,304–946,312 (-), identity 94.4%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 945,552–945,719 (-), identity 30.357%, search hit onlyCIMG_00407MAT1-1-3: core MAT gene, 946,827–947,903 (+), identity 36.5%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 946,827–947,903 (+), identity 90.1%, search hit onlyMAT1-2-1APN2: conserved flank, 948,815–951,002 (-), identity 90.9%, search hit onlyAPN2COX13: conserved flank, 951,362–952,103 (+), identity 94.9%, search hit onlyCOX13
Gene order on MSZS01000006. Scroll sideways to see the whole figure.
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
MSZS01000006:938,973–952,103 · 13.1 kb
Own genes
MSZS01000006:938,973–952,103 · 13.1 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
939.0 kb
Region (samtools)
MSZS01000006:938973-952103
Idiomorph scores
  • MAT1-2 573.0

Margin over the set-aside MAT1-1-3 hit: 53.6 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-190.1MAT1-1-336.5100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome97.7cov 88.7%< 1e-1801,738 bits938,973–942,872 +–746128_a1163_MAT_MAT1-1P174DRAFT_443607
MAT1-2-4core MAThit onlyDIAMOND, proteome94.4cov 99.2%4.9e-175476 bits945,304–946,312 -–746128_af293_MAT_MAT1-2P174DRAFT_422793
CIMG_00407core MAThit onlytblastn30.4cov 21.0%1.2e-0737 bits945,552–945,719 -–5501_rs_MAT_MAT1-2P174DRAFT_422793
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome36.5cov 41.1%3.7e-1154 bits946,827–947,903 +–230073_uamh-1363_MAT_MAT1-1P174DRAFT_462167
MAT1-2-1core MAThit onlyDIAMOND, proteome90.1cov 100.0%2.2e-211573 bits946,827–947,903 +–746128_af293_MAT_MAT1-2P174DRAFT_462167
APN2conserved flankhit onlyDIAMOND, proteome90.9cov 36.7%< 1e-1801,152 bits948,815–951,002 -–2903220_liq80xsp_MAT_combinedP174DRAFT_443610
COX13conserved flankhit onlyDIAMOND, proteome94.9cov 100.0%5.0e-91253 bits951,362–952,103 +–746128_af293_MAT_MAT1-2P174DRAFT_244239

Withheld candidate loci 1

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (1).

Show the 1 withheld locus
familylocationidiomorph genesmodelledreason
MATMSZS01000005:1,167,349–1,169,524MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
AnovofumigatusIBT16806.genome.fasta
Assembly
62 contigs · 32.4 Mb · N50 3.8 Mb
Genome SHA-256
1ce16634f74ef9c88d25576314b5f6ad77d12a3634bc1bc28ee5218f43643a25
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:05:24Z
Run time
66 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits