MATPredict · mating-type report

AnigerN402ATCC64974

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 02:05 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on OGUI01000016.1MAT1-1 locus: genes from left to right on OGUI01000016.1, 2,517,304 to 2,548,889: MAT1-1-1 (core MAT gene, minus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand); MAT1-1-3 (core MAT gene, minus strand); SLA2 (conserved flank, minus strand).2,520 kb2,525 kb2,530 kb2,535 kb2,540 kb2,545 kb2,550 kb349.1 kb more to contig end →called locusMAT1-1-1: core MAT gene, 2,517,304–2,518,461 (-), identity 50.4%, search hit onlyMAT1-1-1APN2: conserved flank, 2,520,742–2,522,857 (-), identity 90.9%, search hit onlyAPN2COX13: conserved flank, 2,523,413–2,524,128 (+), identity 77.3%, search hit onlyCOX13MAT1-1-3: core MAT gene, 2,531,902–2,531,994 (-), identity 41.935%, search hit onlyMAT1-1-3SLA2: conserved flank, 2,545,065–2,548,889 (-), identity 90.1%, search hit onlySLA2
Gene order on OGUI01000016.1. Scroll sideways to see the whole figure.
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
OGUI01000016.1:2,517,304–2,548,889 · 31.6 kb
Own genes
OGUI01000016.1:2,517,304–2,548,889 · 31.6 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
4 of 5 gene hits
Nearest contig end
350.7 kb
Region (samtools)
OGUI01000016.1:2517304-2548889
Idiomorph scores
  • MAT1-1 330.0

No other idiomorph scored.

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-1-1core MAThit onlyDIAMOND, proteome50.4cov 93.9%3.4e-114330 bits2,517,304–2,518,461 -–162425_fgsc-a4_MAT_combinedATCC64974_94950
APN2conserved flankhit onlyDIAMOND, proteome90.9cov 36.7%< 1e-180884 bits2,520,742–2,522,857 -–2903220_liq80xsp_MAT_combinedATCC64974_94960
COX13conserved flankhit onlyDIAMOND, proteome77.3cov 100.0%1.8e-74211 bits2,523,413–2,524,128 +–746128_af293_MAT_MAT1-2ATCC64974_94970
MAT1-1-3core MAThit onlytblastn41.9cov 17.0%7.8e+0027 bits2,531,902–2,531,994 -–5518_3639_MAT_combinednone
SLA2conserved flankhit onlyDIAMOND, proteome90.1cov 100.0%< 1e-1801,591 bits2,545,065–2,548,889 -–746128_af293_MAT_MAT1-2ATCC64974_95040

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci
familylocationidiomorph genesmodelledreason
MATOGUI01000011.1:544,151–546,206MAT1-2MAT1-2-11too few of the family's genes found
MATOGUI01000013.1:2,376,577–2,378,667MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
AnigerN402ATCC64974.genome.fasta
Assembly
19 contigs · 35.6 Mb · N50 2.9 Mb
Genome SHA-256
e53c318dfeaee3bf4e94602bf9352bf7f62f861c964769ec50403af38f2764f0
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'genus', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T02:03:53Z
Run time
79 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits