MATPredict · mating-type report

AellipticusCBS707.79

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:59 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2high confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on KZ826050MAT1-2 locus: genes from left to right on KZ826050, 4,113 to 11,201: MAT1-2-4 (core MAT gene, minus strand); CIMG_00407 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).4 kb6 kb8 kb10 kb← 3.8 kb more to contig endcalled locusMAT1-2-4: core MAT gene, 4,113–5,032 (-), identity 45.8%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 4,189–4,365 (-), identity 30.508%, search hit onlyCIMG_00407MAT1-1-3: core MAT gene, 5,541–6,684 (+), identity 36.0%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 5,541–6,684 (+), identity 52.3%, search hit onlyMAT1-2-1APN2: conserved flank, 7,885–10,016 (-), identity 88.6%, search hit onlyAPN2COX13: conserved flank, 10,446–11,201 (+), identity 81.1%, search hit onlyCOX13
Gene order on KZ826050. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on KZ826050, 3,759 to 11,555BO71DRAFT_390402 3,643-5,188 (-): putative mating locus proteinBO71DRAFT_390402BO71DRAFT_390405 5,437-6,684 (+): mating-type HMG-box protein MAT1-2BO71DRAFT_390405BO71DRAFT_390410 7,804-10,155 (-): DNA lyase Apn2BO71DRAFT_390410BO71DRAFT_364229 10,402-11,543 (+): putative cytochrome c oxidase subunit VIaBO71DRAFT_364229
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KZ826050:4,113–11,201 · 7.1 kb
Own genes
KZ826050:4,113–11,201 · 7.1 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
4 of 6 gene hits
Nearest contig end
4.1 kb
Region (samtools)
KZ826050:4113-11201
Idiomorph scores
  • MAT1-2 287.0

Margin over the set-aside MAT1-1-3 hit: 16.3 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-152.3MAT1-1-336.0100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-2-4core MAThit onlyDIAMOND, proteome45.8cov 96.0%7.3e-73216 bits4,113–5,032 -–746128_af293_MAT_MAT1-2BO71DRAFT_390402
CIMG_00407core MAThit onlytblastn30.5cov 16.0%1.6e-0134 bits4,189–4,365 -–5501_rs_MAT_MAT1-2BO71DRAFT_390402
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome36.0cov 41.7%1.9e-1464 bits5,541–6,684 +–230073_uamh-1363_MAT_MAT1-1BO71DRAFT_390405
MAT1-2-1core MAThit onlyDIAMOND, proteome52.3cov 58.6%2.9e-98287 bits5,541–6,684 +–199306_silveira_MAT_MAT1-2BO71DRAFT_390405
APN2conserved flankhit onlyDIAMOND, proteome88.6cov 36.7%< 1e-180884 bits7,885–10,016 -–2903220_liq80xsp_MAT_combinedBO71DRAFT_390410
COX13conserved flankhit onlyDIAMOND, proteome81.1cov 78.4%3.5e-62180 bits10,446–11,201 +–162425_fgsc-a4_MAT_combinedBO71DRAFT_364229
SLA2expected, not found–

Withheld candidate loci 3

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (3).

Show the 3 withheld loci

Withheld loci (3)

familylocationidiomorph genesmodelledreason
MATKZ825840:166,764–191,352MAT1-2MAT1-1-1, MAT1-2-11too few of the family's genes found
MATKZ826050:44,841–48,653undeterminedSLA21too few of the family's genes found
MATKZ826059:595–4,407undeterminedSLA21too few of the family's genes found

Provenance

Genome file
AellipticusCBS707.79.genome.fasta
Assembly
518 contigs · 42.9 Mb · N50 159.0 kb
Genome SHA-256
24a3f4bb9de32d735e48c2f26c1caca3a51ee588642dd55bfb0aff8d06b9e7f0
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['high_N', 'identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:57:34Z
Run time
98 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits