MATPredict · mating-type report

AcristatusGZAAS20.1005

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:57 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-2high confidence

Called loci 1

MAT1-2 MAT family · Ascomycota

high confidence
Gene order of the MAT1-2 locus on JXNT01000006MAT1-2 locus: genes from left to right on JXNT01000006, 907,105 to 918,218: SLA2 (conserved flank, plus strand); MAT1-2-4 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).908 kb910 kb912 kb914 kb916 kb918 kb458.4 kb more to contig end →called locusSLA2: conserved flank, 907,105–910,642 (+), identity 89.5%, search hit onlySLA2MAT1-2-4: core MAT gene, 911,521–912,666 (-), identity 40.1%, search hit onlyMAT1-2-4MAT1-1-3: core MAT gene, 913,186–914,373 (+), identity 41.3%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 913,186–914,373 (+), identity 58.5%, search hit onlyMAT1-2-1APN2: conserved flank, 915,039–917,133 (-), identity 88.6%, search hit onlyAPN2COX13: conserved flank, 917,461–918,218 (+), identity 71.4%, search hit onlyCOX13
Gene order on JXNT01000006. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on JXNT01000006, 906,551 to 918,773SI65_06275 907,105-910,642 (+): unspecified productSI65_06275SI65_06276 911,521-912,666 (-): unspecified productSI65_06276SI65_06277 913,186-914,373 (+): HMG box domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A1B0THQ3]SI65_06277SI65_06278 915,039-917,133 (-): Endo/exonuclease/phosphatase domain-containing protein [Source:UniProtKB/TrEMBL;Acc:A0A1E3BBS1]SI65_06278SI65_06279 917,461-918,218 (+): unspecified productSI65_06279SI65_06280 918,615-921,231 (-): Anaphase-promoting complex subunit 5 [Source:UniProtKB/TrEMBL;Acc:A0A1E3BBU9]SI65_06280
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
JXNT01000006:907,105–918,218 · 11.1 kb
Own genes
JXNT01000006:907,105–918,218 · 11.1 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 6 gene hits
Nearest contig end
458.9 kb
Region (samtools)
JXNT01000006:907105-918218
Idiomorph scores
  • MAT1-2 305.0

Margin over the set-aside MAT1-1-3 hit: 17.2 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-158.5MAT1-1-341.3100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome89.5cov 100.0%< 1e-1801,592 bits907,105–910,642 +–107463_unknown-1_MAT_MAT1-2SI65_06275
MAT1-2-4core MAThit onlyDIAMOND, proteome40.1cov 96.8%4.5e-51162 bits911,521–912,666 -–746128_af293_MAT_MAT1-2SI65_06276
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome41.3cov 41.7%1.1e-1464 bits913,186–914,373 +–230073_uamh-1363_MAT_MAT1-1SI65_06277
MAT1-2-1core MAThit onlyDIAMOND, proteome58.5cov 65.1%3.4e-105305 bits913,186–914,373 +–61372_suf959_MAT_MAT1-2SI65_06277
APN2conserved flankhit onlyDIAMOND, proteome88.6cov 36.7%6.0e-304833 bits915,039–917,133 -–2903220_liq80xsp_MAT_combinedSI65_06278
COX13conserved flankhit onlyDIAMOND, proteome71.4cov 100.0%5.0e-68195 bits917,461–918,218 +–746128_af293_MAT_MAT1-2SI65_06279

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATJXNT01000001:2,233,120–2,235,329MAT1-2MAT1-2-11too few of the family's genes found
MATJXNT01000005:1,075,715–1,078,383MAT1-1MAT1-1-1, MAT1-2-4, CIMG_004072too few of the family's genes found

Provenance

Genome file
AcristatusGZAAS20.1005.genome.fasta
Assembly
68 contigs · 28.5 Mb · N50 2.3 Mb
Genome SHA-256
3b4a35805aaa4406caa172e93c6daf0c3d30db5eb138f242b617a770260774b9
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:56:21Z
Run time
70 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits