MATPredict · mating-type report

AclavatusNRRL1

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:56 UTC by MATPredict 0.8.1

Result

Mating type

MAT1-1high confidence

Called loci 1

MAT1-1 MAT family · Ascomycota

high confidence
Gene order of the MAT1-1 locus on DS027056MAT1-1 locus: genes from left to right on DS027056, 267,239 to 280,431: SLA2 (conserved flank, plus strand); MAT1-1-1 (core MAT gene, minus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand).270 kb275 kb280 kb← 266.6 kb more to contig endcalled locusSLA2: conserved flank, 267,239–271,215 (+), identity 91.8%, search hit onlySLA2MAT1-1-1: core MAT gene, 273,880–275,050 (-), identity 58.5%, search hit onlyMAT1-1-1MAT1-2-1: core MAT gene, 275,520–276,192 (+), identity 43.6%, search hit onlyMAT1-2-1APN2: conserved flank, 277,114–279,295 (-), identity 93.2%, search hit onlyAPN2COX13: conserved flank, 279,677–280,431 (+), identity 81.6%, search hit onlyCOX13
Gene order on DS027056. Scroll sideways to see the whole figure.
FungiDB release 68 gene models on DS027056, 266,579 to 281,090ACLA_034100 267,239-271,215 (+): ANTH domain proteinACLA_034100ACLA_034110 273,880-275,050 (-): MAT alpha mating-type protein, putativeACLA_034110ACLA_034120 275,520-276,192 (+): mating-type HMG box protein partial, putativeACLA_034120ACLA_034130 277,114-279,295 (-): ap endonucleaseACLA_034130ACLA_034140 279,677-280,431 (+): cytochrome c oxidase subunit VIa, putativeACLA_034140ACLA_034150 280,844-283,475 (-): anaphase-promoting complex proteinACLA_034150
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
Location
DS027056:267,239–280,431 · 13.2 kb
Own genes
DS027056:267,239–280,431 · 13.2 kb
Locus
Genes of both idiomorphs at one locus.
Genes modelled
5 of 5 gene hits
Nearest contig end
267.2 kb
Region (samtools)
DS027056:267239-280431
Idiomorph scores
  • MAT1-1 397.0
  • MAT1-2 95.9

Margin over the next idiomorph: 301.1

Score: bitscore of the best core-gene hit for each idiomorph.

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
SLA2conserved flankhit onlyDIAMOND, proteome91.8cov 100.0%< 1e-1801,683 bits267,239–271,215 +–746128_af293_MAT_MAT1-2ACLA_034100
MAT1-1-1core MAThit onlyDIAMOND, proteome58.5cov 99.7%2.5e-140397 bits273,880–275,050 -–746128_a1163_MAT_MAT1-1ACLA_034110
MAT1-2-1core MAThit onlyDIAMOND, proteome43.6cov 34.2%1.9e-2796 bits275,520–276,192 +–746128_af293_MAT_MAT1-2ACLA_034120
APN2conserved flankhit onlyDIAMOND, proteome93.2cov 36.7%< 1e-180947 bits277,114–279,295 -–2903220_liq80xsp_MAT_combinedACLA_034130
COX13conserved flankhit onlyDIAMOND, proteome81.6cov 100.0%7.3e-77217 bits279,677–280,431 +–746128_af293_MAT_MAT1-2ACLA_034140

Withheld candidate loci 2

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (2).

Show the 2 withheld loci

Withheld loci (2)

familylocationidiomorph genesmodelledreason
MATDS027054:1,426,644–1,428,845MAT1-2MAT1-2-11too few of the family's genes found
MATDS027054:1,895,526–1,895,936MAT1-1MAT1-1-1, MAT1-1-31too few of the family's genes found

Provenance

Genome file
AclavatusNRRL1.genome.fasta
Assembly
144 contigs · 27.9 Mb · N50 2.5 Mb
Genome SHA-256
c1ab23e49ccb84b73f5c6a96c242f1bcf81aacbdbdf561e3ddfb63cf603975dc
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': [], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:55:12Z
Run time
67 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits