MATPredict · mating-type report

AbrasiliensisCBS101740

MAT locus above the FungiDB annotation (gene by gene)

organism not recorded

Run by MATPredict 0.8.1 (v0.8.1, 3a62e6302b) · database 86f18d232e4f · local NCBI taxonomy snapshot 2026-10-01 · rendered 2026-11-07 01:52 UTC by MATPredict 0.8.1

Result

Mating type

Called loci 1

MAT1-2 MAT family · Ascomycota

medium confidence
Gene order of the MAT1-2 locus on KV878679MAT1-2 locus: genes from left to right on KV878679, 4,244,901 to 4,278,419: MAT1-2-4 (core MAT gene, minus strand); CIMG_00407 (core MAT gene, minus strand); MAT1-1-3 (core MAT gene, plus strand); MAT1-2-1 (core MAT gene, plus strand); APN2 (conserved flank, minus strand); COX13 (conserved flank, plus strand); SLA2 (conserved flank, minus strand).4,250 kb4,260 kb4,270 kb4,280 kb256.0 kb more to contig end →called locusMAT1-2-4: core MAT gene, 4,244,901–4,245,749 (-), identity 45.3%, search hit onlyMAT1-2-4CIMG_00407: core MAT gene, 4,245,018–4,245,530 (-), identity 22.778%, search hit onlyCIMG_00407MAT1-1-3: core MAT gene, 4,246,236–4,247,351 (+), identity 32.1%, search hit onlyMAT1-1-3 (lost to MAT1-2-1)MAT1-2-1: core MAT gene, 4,246,236–4,247,351 (+), identity 60.7%, search hit onlyMAT1-2-1APN2: conserved flank, 4,248,387–4,250,521 (-), identity 90.9%, search hit onlyAPN2COX13: conserved flank, 4,250,945–4,251,658 (+), identity 75.9%, search hit onlyCOX13SLA2: conserved flank, 4,274,600–4,278,419 (-), identity 89.8%, search hit onlySLA2
Gene order on KV878679. Scroll sideways to see the whole figure.
FungiDB release 68 annotation of the same window: gene models from the GFF (filled = CDS, pale = UTR exon, line = intron, arrow = strand). Green genes overlap a MATPredict gene on the same strand; grey genes do not. Click a gene to open it on fungidb.org.
  • core MAT gene
  • conserved flank
  • search hit only
  • MAT1-1-3 is a weak hit to the other idiomorph's gene and was not counted against the call.
Location
KV878679:4,244,901–4,278,419 · 33.5 kb
Own genes
KV878679:4,244,901–4,278,419 · 33.5 kb
Locus
Complete locus: MAT gene(s) with their flanking genes.
Genes modelled
5 of 7 gene hits
Nearest contig end
257.6 kb
Region (samtools)
KV878679:4244901-4278419
Idiomorph scores
  • MAT1-2 309.0

Margin over the set-aside MAT1-1-3 hit: 28.6 identity points (the narrowest overlap resolution below).

Score: bitscore of the best core-gene hit for each idiomorph.

Overlapping hits resolved

Where genes of two idiomorphs hit the same place, the stronger hit was kept.

keptidentity %set asideidentity %overlap %basis
MAT1-2-160.7MAT1-1-332.1100.0higher identity

Gene evidence

GeneRoleModel Identity %E-value Position (strand)Exons Reference recordFungiDB gene
MAT1-2-4core MAThit onlyDIAMOND, proteome45.3cov 89.2%4.9e-63191 bits4,244,901–4,245,749 -–746128_af293_MAT_MAT1-2ASPBRDRAFT_25894
CIMG_00407core MAThit onlytblastn22.8cov 57.0%5.6e-0441 bits4,245,018–4,245,530 -–5501_rs_MAT_MAT1-2ASPBRDRAFT_25894
MAT1-1-3core MATother idiomorph, not countedlost to MAT1-2-1, not countedhit onlyDIAMOND, proteome32.1cov 58.9%1.6e-1361 bits4,246,236–4,247,351 +–230073_uamh-1363_MAT_MAT1-1ASPBRDRAFT_167991
MAT1-2-1core MAThit onlyDIAMOND, proteome60.7cov 25.4%3.6e-107309 bits4,246,236–4,247,351 +–115814_voucher-101_MAT_MAT1-2ASPBRDRAFT_167991
APN2conserved flankhit onlyDIAMOND, proteome90.9cov 36.7%< 1e-180913 bits4,248,387–4,250,521 -–2903220_liq80xsp_MAT_combinedASPBRDRAFT_37526
COX13conserved flankhit onlyDIAMOND, proteome75.9cov 100.0%1.8e-74211 bits4,250,945–4,251,658 +–746128_af293_MAT_MAT1-2ASPBRDRAFT_112056
SLA2conserved flankhit onlyDIAMOND, proteome89.8cov 100.0%< 1e-1801,588 bits4,274,600–4,278,419 -–746128_af293_MAT_MAT1-2ASPBRDRAFT_61705

Withheld candidate loci 1

Places with some MAT-gene evidence that did not pass a quality gate. Most genomes have several. They are listed so a known locus can be checked against them, not because they are likely calls.

Reasons: too few of the family's genes found (1).

Show the 1 withheld locus
familylocationidiomorph genesmodelledreason
MATKV878685:439,442–441,638MAT1-2MAT1-2-11too few of the family's genes found

Provenance

Genome file
AbrasiliensisCBS101740.genome.fasta
Assembly
103 contigs · 35.8 Mb · N50 2.0 Mb
Genome SHA-256
881c614d1c1e3b212ed4abc5434e59ec357536d2569f57dccd2748e86346a3b1
MATPredict
0.8.1 (v0.8.1, 3a62e6302b)
Database
86f18d232e4fe95d · 185 records
Taxonomy
local NCBI taxonomy snapshot 2026-10-01
Parameters
phylum=Ascomycota, phylum_guess={'requested': 'auto', 'status': 'ok', 'usable': True, 'reason': None, 'phylum': 'Ascomycota', 'phylum_taxid': 4890, 'deepest_rank': 'species', 'routing_taxid': 1131492, 'routing_taxid_rank': 'family', 'flags': ['identical_to_reference'], 'gmg_version': 'gmg 0.3.2 (66055154a)', 'index': 'bfd-reps-2026.10.5-s20000.gmgi', 'index_sha256': '0f319567f1cab17643e1daf7ffdc0e3b312bd43913e351568108d67a4a19702c', 'index_spec': 'bfd-reps-2026.10.5-s20000.gmgi', 'note': 'GmG called phylum Ascomycota', 'action': 'routed', 'weak_call_accepted': False, 'genetic_code': 1, 'genetic_code_from': 'family taxid 1131492', 'routed_by': 'family taxid 1131492 (lineage)'}, genetic_code=1, min_hits=2, require_core_role=True, max_polished_clusters_per_family=6, polish_strong_identity=50.0, polish_core_pair=True, partial_core_policy=off, homothallic_policy=off, confidence_cap_policy=off, cross_family_policy=off, fallback_after_withheld_policy=off, strong_single_gene_policy=off, scoped_roster_policy=off, scoped_roster=off: BGL1,GAP1 not searched, supported_min_bitscore=33.0
Run started
2026-11-07T01:50:58Z
Run time
79 s
Files
detection_report.yaml, detected_loci.gff3

Cite MATPredict as given in CITATION.cff at github.com/stajichlab/MATPredict. The reference records named in the gene tables are curated database entries; each record's metadata cites its source publication.

How to read this report

Idiomorph
The form of the mating-type locus a strain carries (for example MAT1-1 or MAT1-2, Plus or Minus). Basidiomycota HD and PR alleles are named after the reference they match.
High confidence
Every expected core MAT gene was found and modelled, with a conserved flanking gene where the family has one, on one contig.
Medium confidence
The core genes were found, but a gene could not be modelled, no conserved flank was found, or the locus is split (one step down).
Low confidence
A single isolated gene hit, or no core MAT gene.
Model status
Gene model: the reference protein was aligned to the genome to build the gene (exonerate, miniprot). Models differ: the two tools disagree on the structure; the alternate model is shown. Hit only: a search hit with no model, weaker evidence.
Identity
Percent amino-acid identity between the gene (or hit) and the closest reference protein.
Reference record
The curated database record whose protein matched best. Each record's metadata cites the publication or deposit it comes from.

Limits